中国继续医学教育
中國繼續醫學教育
중국계속의학교육
China Continuing Medical Education
2015年
24期
45-47
,共3页
张兴利%吴鹏飞%邱宏春%孔荣
張興利%吳鵬飛%邱宏春%孔榮
장흥리%오붕비%구굉춘%공영
原发免疫性血小板减少症%白细胞介素18%CXC趋化因子配体10
原髮免疫性血小闆減少癥%白細胞介素18%CXC趨化因子配體10
원발면역성혈소판감소증%백세포개소18%CXC추화인자배체10
Primary immune thrombocytopenia%Interleukin-18%CXC chemo-kine ligand-10
目的：研究原发免疫性血小板减少症（primaryimmunethrombocytopenia， ITP）患者血清中白细胞介素-18（Interleukin-18，IL-18）、CXC趋化因子配体10（CXC chemo-kine ligand-10，CXCL10）的表达水平，探讨其在ITP发病中的临床意义。方法采用酶联免疫吸附法（enzyme-linked immunosorbent assay，ELISA）定量检测45例ITP患者、20例健康者（正常对照组）血清IL-18、CXCL10的水平。结果激素治疗前ITP患者血清IL-18、CXCL10的表达水平均高于正常对照组（t=5.126，6.576，P＜0.05），治疗后两者与治疗前比较均有统计学差异（t=10.616，7.399，P＜0.05）。激素治疗有效组IL-18、CXCL10的表达水平与正常对照组比较无统计学差异（q=0.521，0.871，P＞0.05），而无效组IL-18的表达水平仍高于有效组和正常对照组（q=6.137，5.867， P＜0.05）；无效组CXCL10的表达水平同样高于有效组和正常对照组（q=8.933，7.642，P＜0.05）。初发未治疗ITP患者血清IL-18、CXCL10表达呈正相关性（r=0.539，P＜0.05）。结论 ITP患者不同时期血清中IL-18、CXCL10的表达水平不同，且两者具有显著相关性，提示其可能在ITP的发病及治疗过程中发挥一定的作用，联合检测有望在ITP的病情监测、预后判断等方面提供依据。
目的：研究原髮免疫性血小闆減少癥（primaryimmunethrombocytopenia， ITP）患者血清中白細胞介素-18（Interleukin-18，IL-18）、CXC趨化因子配體10（CXC chemo-kine ligand-10，CXCL10）的錶達水平，探討其在ITP髮病中的臨床意義。方法採用酶聯免疫吸附法（enzyme-linked immunosorbent assay，ELISA）定量檢測45例ITP患者、20例健康者（正常對照組）血清IL-18、CXCL10的水平。結果激素治療前ITP患者血清IL-18、CXCL10的錶達水平均高于正常對照組（t=5.126，6.576，P＜0.05），治療後兩者與治療前比較均有統計學差異（t=10.616，7.399，P＜0.05）。激素治療有效組IL-18、CXCL10的錶達水平與正常對照組比較無統計學差異（q=0.521，0.871，P＞0.05），而無效組IL-18的錶達水平仍高于有效組和正常對照組（q=6.137，5.867， P＜0.05）；無效組CXCL10的錶達水平同樣高于有效組和正常對照組（q=8.933，7.642，P＜0.05）。初髮未治療ITP患者血清IL-18、CXCL10錶達呈正相關性（r=0.539，P＜0.05）。結論 ITP患者不同時期血清中IL-18、CXCL10的錶達水平不同，且兩者具有顯著相關性，提示其可能在ITP的髮病及治療過程中髮揮一定的作用，聯閤檢測有望在ITP的病情鑑測、預後判斷等方麵提供依據。
목적：연구원발면역성혈소판감소증（primaryimmunethrombocytopenia， ITP）환자혈청중백세포개소-18（Interleukin-18，IL-18）、CXC추화인자배체10（CXC chemo-kine ligand-10，CXCL10）적표체수평，탐토기재ITP발병중적림상의의。방법채용매련면역흡부법（enzyme-linked immunosorbent assay，ELISA）정량검측45례ITP환자、20례건강자（정상대조조）혈청IL-18、CXCL10적수평。결과격소치료전ITP환자혈청IL-18、CXCL10적표체수평균고우정상대조조（t=5.126，6.576，P＜0.05），치료후량자여치료전비교균유통계학차이（t=10.616，7.399，P＜0.05）。격소치료유효조IL-18、CXCL10적표체수평여정상대조조비교무통계학차이（q=0.521，0.871，P＞0.05），이무효조IL-18적표체수평잉고우유효조화정상대조조（q=6.137，5.867， P＜0.05）；무효조CXCL10적표체수평동양고우유효조화정상대조조（q=8.933，7.642，P＜0.05）。초발미치료ITP환자혈청IL-18、CXCL10표체정정상관성（r=0.539，P＜0.05）。결론 ITP환자불동시기혈청중IL-18、CXCL10적표체수평불동，차량자구유현저상관성，제시기가능재ITP적발병급치료과정중발휘일정적작용，연합검측유망재ITP적병정감측、예후판단등방면제공의거。
Objective To study the expression of Interleukin-18 (IL-18) and CXC chemo-kine ligand-10 (CXCL10) in primary immune thrombocytopenia (ITP) and clarify their clinical significance. Methods Enzyme-linked immunosorbent assay (ELISA) was used to detect the expression of IL-18 and CXCL10 in serum samples from 45 ITP patients, 20 volunteers (normal control group) served as controls. Results The expression levels of IL-18 and CXCL10 in ITP untreated group were significantly higher than normal control group (t=5.126, 6.576, P<0.05). Both IL-18 and CXCL10 of post-treatment group were significantly different with untreated group (t=10.616, 7.399, P<0.05). Additionally, the levels of IL-18 and CXCL10 in effective-treatment group were not significantly different with normal control (q=0.521, 0.871, P>0.05). However, the levels of IL-18 in ineffective-treatment group were still signiifcantly higher than effective-treatment group and normal control group (q=6.137, 5.867, P<0.05). The levels of CXCL10 in ineffective-treatment group were also significantly higher than effective-treatment group and normal control group (q=8.933, 7.642, P<0.05). There was positive correlation between serum expression levels of IL-18 and CXCL10 in ITP untreated group (r=0.539, P<0.05). Conclusion Different expressions of serum IL-18 and CXCL10 were found in ITP patients, which relfected they may play an important role in pathogenesis and therapy of ITP. United detection of serum IL-18 and CXCL10 may be used as reference indexes of ITP’ clinical stages and prognosis.
