CAJ | 학술논문

目的：建立 HPLC‐MS测定大鼠血浆中丹酚酸B质量浓度的方法，预测盐酸右美托咪定对丹酚酸B在大鼠体内代谢的影响。方法将SD大鼠随机分成丹酚酸B组和丹酚酸B＋盐酸右美托咪定组，采用 HPLC‐MS法对大鼠体内丹酚酸B血药质量浓度进行测定。结果丹酚酸B血浆加标样品在0．01～10μg · m L －1内线性关系良好（ r＝0．9978）；丹酚酸B高、中、低质量浓度回收率均在84％～98％之间。与丹酚酸B组比，丹酚酸B＋盐酸右美托咪定组中丹酚酸B清除速率（CL）有所降低，药时曲线下面积（AUC）有所增加，但均无显著性影响（P＞0．05）。结论在大鼠体内盐酸右美托咪定对丹酚酸B代谢无明显影响。
목적：건립 HPLC‐MS측정대서혈장중단분산B질량농도적방법，예측염산우미탁미정대단분산B재대서체내대사적영향。방법장SD대서수궤분성단분산B조화단분산B＋염산우미탁미정조，채용 HPLC‐MS법대대서체내단분산B혈약질량농도진행측정。결과단분산B혈장가표양품재0．01～10μg · m L －1내선성관계량호（ r＝0．9978）；단분산B고、중、저질량농도회수솔균재84％～98％지간。여단분산B조비，단분산B＋염산우미탁미정조중단분산B청제속솔（CL）유소강저，약시곡선하면적（AUC）유소증가，단균무현저성영향（P＞0．05）。결론재대서체내염산우미탁미정대단분산B대사무명현영향。
Objective To establish an HPLC‐MS method for the determination of salvianolic acid B in rat plasma and to obtain the in‐formation about the influence of dexmedetomidine hydrochloride on the metabolism of salvianolic acid B in vivo .Methods The rats were divided into salvianolic acid B group and salvianolic acid B+dexmedetomidine hydrochloride group randomly .Plasma concen‐tration of salvianolic acid B was determinated by HPLC‐MS after oral administration .Results The calibration curve was linear in the range of 0 .01‐10 .00 μg · mL -1 for salvianolic acid B (r=0 .997 8) .The average recoveries of salvianolic acid B at high ,medi‐um ,and low concentration were between 84% and 98% .Compared with salvianolic acid B group ,in salvianolic acid B+ dexme‐detomidine hydrochloride group ,clearance rate (CL) decreased and area under the curve (AUC) increased slightly ,but there was no significant difference(P> 0 .05) .Conclusion Dexmedetomidine hydrochloride had no effect on the metabolism of salvianolic acid B in rat .No drug‐interaction was observed.