CAJ | 학술논문

目的：考察酸枣仁汤（SZRT ）对睡眠剥夺大鼠认知功能和胆碱能系统的影响。方法雄性大鼠随机分为5组即大平台对照组、睡眠剥夺组及SZRT高、中、低剂量组，釆用改良多平台法建立大鼠睡眠剥夺模型、Y型电迷宫测定学习记忆、HE染色观察大鼠海马组织形态学、比色法测定海马Ach含量及AChE与ChA T的活性。结果与睡眠剥夺组相比，Y‐迷宫，SZRT 高剂量组错误次数减少，主动回避率增多。SZRT可减轻睡眠剥夺大鼠海马锥体细胞损伤，酸枣仁汤高、中剂量组可提高海马Ach含量，降低AChE活性，提高ChAT活性。结论 SZRT能改善睡眠剥夺大鼠所致的学习记忆下降，可能与保护胆碱能系统有关。
목적：고찰산조인탕（SZRT ）대수면박탈대서인지공능화담감능계통적영향。방법웅성대서수궤분위5조즉대평태대조조、수면박탈조급SZRT고、중、저제량조，변용개량다평태법건립대서수면박탈모형、Y형전미궁측정학습기억、HE염색관찰대서해마조직형태학、비색법측정해마Ach함량급AChE여ChA T적활성。결과여수면박탈조상비，Y‐미궁，SZRT 고제량조착오차수감소，주동회피솔증다。SZRT가감경수면박탈대서해마추체세포손상，산조인탕고、중제량조가제고해마Ach함량，강저AChE활성，제고ChAT활성。결론 SZRT능개선수면박탈대서소치적학습기억하강，가능여보호담감능계통유관。
Objective To investigate the effects of Suanzaoren Decoction (SZRT ) on cognitive function and cholinergic system in sleep deprived rats .Methods Rats were randomly divided into 5 groups ,big platform control group ,sleep deprived group ,high ,medium and low dose group of SZRT .The rat model of sleep deprived was established with modified multiple platform method .Learning and memory of rats were tested by Y‐type maze .The histomorphology in hippocampal was observed by HE staining .The contents of acetylcholine ,activity of choline acetyltransferase and cholinesterase of rat hippocampal were determined by colorimetry .Results Compared with sleep deprived group ,high‐dose group can significantly decrease the error number and increase the active avoid‐ance .SZRT can reduce the hippocampal pyramidal cells injury .High and medium‐dose group can obviously increase the content of acetylcholine ,increase cholinesterase and decrease choline acetyltransferase activity in hippocampal .Conclusion SZRT can im‐prove the learning and memory in rats after sleep deprivation ,which probably was related to the protection of cholinergic system .