中国继续医学教育
中國繼續醫學教育
중국계속의학교육
China Continuing Medical Education
2015年
24期
211-212
,共2页
树突状细胞疫苗%MB49细胞%膀胱癌模型%免疫组织化学染色
樹突狀細胞疫苗%MB49細胞%膀胱癌模型%免疫組織化學染色
수돌상세포역묘%MB49세포%방광암모형%면역조직화학염색
Dendritic cell vaccine%MB49 cells%Bladder cancer models%Immunohistochemical staining
目的：探讨负载MB49细胞粗提全抗原的树突状细胞（DC2.4）疫苗对C57BL/6小鼠膀胱癌MB49皮下移植瘤模型的抑瘤效果。方法制备负载MB49细胞粗提全抗原的DC疫苗；建立小鼠MB49膀胱癌皮下移植瘤模型。造模后第7 d、14 d，疫苗组小鼠于右前腋皮下注射疫苗，PBS对照组相应注射PBS，正常组正常饲养。造模第21 d，测量肿瘤体积和重量。应用免疫组化染色方法检测肿瘤组织内CD4+、CD8+细胞数。应用SPSS13.0统计分析测量数据。结果 DC疫苗组和PBS对照组小鼠肿瘤体积随时间呈现不同程度增长趋势，DC疫苗组缓于PBS对照组，差异有统计学意义（P＜0.01）；造模21 d，DC疫苗组小鼠肿瘤重量小于PBS对照组，差异有统计学意义（P＜0.01）；免疫组化染色检测小鼠肿瘤组织内CD4+、CD8+T细胞数，DC疫苗组CD4+、CD8+T细胞数高于PBS对照组，差异有统计学意义（P＜0.01）。结论 DC2.4树突状细胞疫苗抑制了模型小鼠肿瘤的生长，起到了抗肿瘤的免疫学效应。
目的：探討負載MB49細胞粗提全抗原的樹突狀細胞（DC2.4）疫苗對C57BL/6小鼠膀胱癌MB49皮下移植瘤模型的抑瘤效果。方法製備負載MB49細胞粗提全抗原的DC疫苗；建立小鼠MB49膀胱癌皮下移植瘤模型。造模後第7 d、14 d，疫苗組小鼠于右前腋皮下註射疫苗，PBS對照組相應註射PBS，正常組正常飼養。造模第21 d，測量腫瘤體積和重量。應用免疫組化染色方法檢測腫瘤組織內CD4+、CD8+細胞數。應用SPSS13.0統計分析測量數據。結果 DC疫苗組和PBS對照組小鼠腫瘤體積隨時間呈現不同程度增長趨勢，DC疫苗組緩于PBS對照組，差異有統計學意義（P＜0.01）；造模21 d，DC疫苗組小鼠腫瘤重量小于PBS對照組，差異有統計學意義（P＜0.01）；免疫組化染色檢測小鼠腫瘤組織內CD4+、CD8+T細胞數，DC疫苗組CD4+、CD8+T細胞數高于PBS對照組，差異有統計學意義（P＜0.01）。結論 DC2.4樹突狀細胞疫苗抑製瞭模型小鼠腫瘤的生長，起到瞭抗腫瘤的免疫學效應。
목적：탐토부재MB49세포조제전항원적수돌상세포（DC2.4）역묘대C57BL/6소서방광암MB49피하이식류모형적억류효과。방법제비부재MB49세포조제전항원적DC역묘；건립소서MB49방광암피하이식류모형。조모후제7 d、14 d，역묘조소서우우전액피하주사역묘，PBS대조조상응주사PBS，정상조정상사양。조모제21 d，측량종류체적화중량。응용면역조화염색방법검측종류조직내CD4+、CD8+세포수。응용SPSS13.0통계분석측량수거。결과 DC역묘조화PBS대조조소서종류체적수시간정현불동정도증장추세，DC역묘조완우PBS대조조，차이유통계학의의（P＜0.01）；조모21 d，DC역묘조소서종류중량소우PBS대조조，차이유통계학의의（P＜0.01）；면역조화염색검측소서종류조직내CD4+、CD8+T세포수，DC역묘조CD4+、CD8+T세포수고우PBS대조조，차이유통계학의의（P＜0.01）。결론 DC2.4수돌상세포역묘억제료모형소서종류적생장，기도료항종류적면역학효응。
Objective To investigate the antitumor effect of dendritic cell vaccine against C57BL/6 cells loaded with MB49 cells in MB49 mice. Methods DC vaccine against MB49 cells were prepared by loading MB49 cells, and the model was established in mice. After making the model, the 7 d, 14 d, the vaccine were injected in right anterior axillary of vaccine group mice, and the mice of PBS control group were injected with PBS, normal feeding to normal group. 21 d after modeling, measurement of the volume and weight of the tumor. The number of CD4+ and CD8+ cells in tumor tissues was detected by immunohistochemical staining. SPSS13.0 statistical analysis of measurement data. Results The tumor volume of DC vaccine group and PBS control group showed different degrees of growth trend, DC vaccine group was signiifcantly slower than that in 21 d control group (P<0.01). The tumor weight was signiifcantly less than that in DC group (P<0.01). In tumor tissue of CD4+and CD8+T cell number and DC vaccine group of CD4+, CD8+T cel number higher than the PBS control group. The difference was statistical y signiifcant (P<0.01). Conclusion DC2.4 dendritic cel vaccine inhibits tumor growth in mouse model, which has the effect of anti-tumor.
