CAJ | 학술논문

目的：建立 HPLC‐MS／MS法测定川楝子中川楝素的方法。方法采用XTerra C18色谱柱（150 mm ×2．1 mm ，5μm），以乙腈‐0．2 mL · L －1甲酸溶液（31∶69）为流动相，采用多反应监测（MRM ）模式进行质谱测定。结果川楝素检出限（LOD）为0．17 ng ，定量限（LOQ ）为0．51 ng ；川楝素质量浓度在0．05～5．00μg · mL －1范围内，与峰面积线性关系良好，r＝0．9999；精密度良好，平均回收率为98．0％，RSD为1．5％。结论该方法具有较好的专属性和灵敏度，能够简便、快速地测定川楝子药材中川楝素的含量。
목적：건립 HPLC‐MS／MS법측정천련자중천련소적방법。방법채용XTerra C18색보주（150 mm ×2．1 mm ，5μm），이을정‐0．2 mL · L －1갑산용액（31∶69）위류동상，채용다반응감측（MRM ）모식진행질보측정。결과천련소검출한（LOD）위0．17 ng ，정량한（LOQ ）위0．51 ng ；천련소질량농도재0．05～5．00μg · mL －1범위내，여봉면적선성관계량호，r＝0．9999；정밀도량호，평균회수솔위98．0％，RSD위1．5％。결론해방법구유교호적전속성화령민도，능구간편、쾌속지측정천련자약재중천련소적함량。
Objective To establish a method of HPLC‐MS/MS for detecting toosendanin (TSN ) in Toosendan Fructus . Methods The analytes were isolated on a XTerra C18 column (150 mm × 2 .1 mm ,5 μm) by isocratic elution with acetonitrile‐mL · L -1formic acid(31∶ 69)as the mobile phase ,and detected by MS/MS in a multiple reaction monitoring (MRM) mode . Results LOD of TSN was 0 .17 ng ,and LOQ of TSN was 0 .51 ng ;The linear range of TSN concentration was 0 .05‐5 .00μg · mL -1 (r=0 .999 9);The average recoveries were 98 .0% with RSD 1 .5% .Conclusion The method is simple and rapid with high sensitivity and good reproducibility ,and it could be used to determine the contents of TSN in Toosendan Fructus .