CAJ | 학술논문

目的：探讨利福平对鱼藤酮诱导的BV2小胶质细胞产生炎症反应的影响及相关机制。方法将经过利福平预处理的BV2小胶质细胞加入鱼藤酮刺激，用Real-time PCR以及酶联免疫吸附试验（ELISA）法检测接受药物处理后小胶质细胞产生IL-1β的变化；采用caspase-1活性检测试剂盒检测小胶质细胞内caspase-1的活性，流式细胞术检测细胞凋亡率。用SPSS 16.0统计软件进行统计分析，计量资料使用均数±标准差（ x-±s）表示，多组间比较采用单因素方差分析，两两比较采用LSD-t检验，P＜0.05说明差异有统计学意义。结果与鱼藤酮组相比，50μmol／L利福平预处理组可明显降低小胶质细胞内鱼藤酮介导的炎症因子IL-1β的基因表达（ t＝4.11，P＜0.05）和上清液中IL-1β的分泌（t＝35.81，P＜0.05），并显著抑制caspase-1的活性（t＝11.62，P＜0.05）。将BV2小胶质细胞与PC12细胞用Transwell体系共培养，利福平预处理组（25、50μmol／L）和caspase-1抑制剂组的PC12细胞的细胞凋亡率均低于鱼藤酮组，差异有统计学意义（ t值分别为3.82、7.98、10.12，均P＜0.05）。结论利福平可能通过抑制caspase-1活化对抗鱼藤酮刺激下小胶质细胞产生的炎症毒性。
목적：탐토리복평대어등동유도적BV2소효질세포산생염증반응적영향급상관궤제。방법장경과리복평예처리적BV2소효질세포가입어등동자격，용Real-time PCR이급매련면역흡부시험（ELISA）법검측접수약물처리후소효질세포산생IL-1β적변화；채용caspase-1활성검측시제합검측소효질세포내caspase-1적활성，류식세포술검측세포조망솔。용SPSS 16.0통계연건진행통계분석，계량자료사용균수±표준차（ x-±s）표시，다조간비교채용단인소방차분석，량량비교채용LSD-t검험，P＜0.05설명차이유통계학의의。결과여어등동조상비，50μmol／L리복평예처리조가명현강저소효질세포내어등동개도적염증인자IL-1β적기인표체（ t＝4.11，P＜0.05）화상청액중IL-1β적분비（t＝35.81，P＜0.05），병현저억제caspase-1적활성（t＝11.62，P＜0.05）。장BV2소효질세포여PC12세포용Transwell체계공배양，리복평예처리조（25、50μmol／L）화caspase-1억제제조적PC12세포적세포조망솔균저우어등동조，차이유통계학의의（ t치분별위3.82、7.98、10.12，균P＜0.05）。결론리복평가능통과억제caspase-1활화대항어등동자격하소효질세포산생적염증독성。
Objective To investigate the effect of rifampicin on inflammatory reaction in rotenone-treated BV2 cells.Methods After pre-incubation with rifampicin ,BV2 cells were treated with rotenone .The mRNA and protein levels of IL-1βwere estimated by real-time PCR and ELISA,the activation of caspase-1 was detected by caspase-1 activity assay kit ,and the cell apoptosis was assessed by flow cytometry .SPSS 16.0 was used for statistical analysis and the data were presented as x-±s.The comparison among groups was analyzed using One-way analysis of variance ( ANOVA) followed by LSD-t test,and P<0.05 indicated that the difference was statistically significant .Results Compared with rotenone group , pretreatment with rifampicin(50 μmol/L)could significantly inhibit the gene expression and protein levels of IL-1β(t=4.11, 35.81,both P<0.05),and significantly suppress the activity of caspase-1(t=11.62,P<0.05).Moreover, after co-incubation of BV2 microglial cells in transwell system ,apoptosis rates of PC12 cells in rifampicin pretreatment groups(25 and 50 μmol/L) and caspase-1 inhibitor group were lower than in rotenone group , and the differences were statistically significant (t values were 3.82,7.98,and 10.12,respectively,all P<0.05).Conclusion Rifampicin may protect BV2 cells against inflammation induced by rotenone via suppressing activation of caspase-1.