华北农学报
華北農學報
화북농학보
Acta Agriculturae Boreali-Sinica
2015年
4期
84-89
,共6页
罗启慧%刘芸%唐秀莹%陈正礼
囉啟慧%劉蕓%唐秀瑩%陳正禮
라계혜%류예%당수형%진정례
大豆异黄酮%肥胖大鼠%免疫组织化学 SABC%原位杂交%α-MSH%POMC%Ob-Rb
大豆異黃酮%肥胖大鼠%免疫組織化學 SABC%原位雜交%α-MSH%POMC%Ob-Rb
대두이황동%비반대서%면역조직화학 SABC%원위잡교%α-MSH%POMC%Ob-Rb
Soy isoflavone%Obese rat%Immunohistochemistry%Hybridization situ%α-MSH%POMC%Ob-Rb
为了探究大豆异黄酮通过瘦素减重的作用机制，建立食源性肥胖大鼠模型，后进行低、中、高剂量（50，150，450 mg ／kg）大豆异黄酮干预，并每周定期称量记录体重，采用苏丹Ⅲ染色、SABC 免疫组织化学法和核酸原位杂交法，观察大豆异黄酮对肥胖大鼠体重、腹部脂肪细胞形态和下丘脑弓状核及垂体α-MSH、POMC mRNA、Ob-Rb mRNA 表达的影响。结果显示，标准对照大鼠α-MSH 在弓状核、垂体神经部和中间部均大量表达，POMC mRNA 在弓状核和垂体仅少量表达，Ob-Rb mRNA 仅在弓状核表达，同时肥胖大鼠3种因子表达均显著降低；大豆异黄酮干预后肥胖大鼠体重降低，腹部脂肪细胞面积缩小且形态分布明显改善，同时α-MSH、POMC mRNA、Ob-Rb mRNA 表达增多。表明大豆异黄酮可能通过减少脂储、增加 Ob-Rb 表达从而促进具有抑食作用的 POMC 及其裂解物α-MSH 产生，从而改善大鼠瘦素抵抗状态，发挥减重作用。
為瞭探究大豆異黃酮通過瘦素減重的作用機製，建立食源性肥胖大鼠模型，後進行低、中、高劑量（50，150，450 mg ／kg）大豆異黃酮榦預，併每週定期稱量記錄體重，採用囌丹Ⅲ染色、SABC 免疫組織化學法和覈痠原位雜交法，觀察大豆異黃酮對肥胖大鼠體重、腹部脂肪細胞形態和下丘腦弓狀覈及垂體α-MSH、POMC mRNA、Ob-Rb mRNA 錶達的影響。結果顯示，標準對照大鼠α-MSH 在弓狀覈、垂體神經部和中間部均大量錶達，POMC mRNA 在弓狀覈和垂體僅少量錶達，Ob-Rb mRNA 僅在弓狀覈錶達，同時肥胖大鼠3種因子錶達均顯著降低；大豆異黃酮榦預後肥胖大鼠體重降低，腹部脂肪細胞麵積縮小且形態分佈明顯改善，同時α-MSH、POMC mRNA、Ob-Rb mRNA 錶達增多。錶明大豆異黃酮可能通過減少脂儲、增加 Ob-Rb 錶達從而促進具有抑食作用的 POMC 及其裂解物α-MSH 產生，從而改善大鼠瘦素牴抗狀態，髮揮減重作用。
위료탐구대두이황동통과수소감중적작용궤제，건립식원성비반대서모형，후진행저、중、고제량（50，150，450 mg ／kg）대두이황동간예，병매주정기칭량기록체중，채용소단Ⅲ염색、SABC 면역조직화학법화핵산원위잡교법，관찰대두이황동대비반대서체중、복부지방세포형태화하구뇌궁상핵급수체α-MSH、POMC mRNA、Ob-Rb mRNA 표체적영향。결과현시，표준대조대서α-MSH 재궁상핵、수체신경부화중간부균대량표체，POMC mRNA 재궁상핵화수체부소량표체，Ob-Rb mRNA 부재궁상핵표체，동시비반대서3충인자표체균현저강저；대두이황동간예후비반대서체중강저，복부지방세포면적축소차형태분포명현개선，동시α-MSH、POMC mRNA、Ob-Rb mRNA 표체증다。표명대두이황동가능통과감소지저、증가 Ob-Rb 표체종이촉진구유억식작용적 POMC 급기렬해물α-MSH 산생，종이개선대서수소저항상태，발휘감중작용。
To study the weight loss mechanism of soy isoflavone dependent on leptin.In the present study,the food-induced obese rats model was established treated with low,medium and high dose soy isoflavone,to investigate the effects of soy isoflavone on body mass,morphology of fat cell inside the abdomen,expression of α-MSH、POMC mRNA、Ob-Rb mRNA in the hypothalamic arcuate nucleus and pituitary,by weighting every week,Sudan Ⅲ stai-ning,immunohistochemical and in situ hybridization analysis.The results showed α-MSH abundantly expressed in the arcuate nucleus,neurohypophysis and pars intermedia,POMC mRNA rarely expressed in the arcuate nucleus and pituitary,and Ob-Rb mRNA only expressed in the arcuate nucleus,simultaneously the three factors were signifi-cantly less present in fat rats.After treated with soybean isoflavones,the body weight of obese rats decreased,the ar-ea of abdominal fat cells reduced and distribution improved significantly,also the expression of α-MSH,POMC mR-NA,Ob-Rb mRNA increased,which could be positively correlated with the dose.Results suggested that soy isofla-vone might reduce fat storage and stimulated Ob-Rb expression,then promoted production of POMC and α-MSH, thus improve the state of leptin resistance in rats,and cause weight loss.
