CAJ | 학술논문

目的：完善柿叶的定性定量方法，便于这一药用资源的质量控制。方法采用薄层层析色谱法（T LC ）对柿叶中槲皮素、山柰酚、齐墩果酸和熊果酸进行定性鉴别；采用高效液相色谱法（H PLC ）对不同来源柿叶中的山柰酚进行定量分析。色谱柱为Wondasil C18‐WR色谱柱（250 mm ×4．6 mm ，5μm）；流动相为甲醇‐2 mL · L －1磷酸溶液（52∶48），流速为1．0 mL · min－1，检测波长为360 nm ，理论板数按山柰酚峰计算应不低于8000。结果 T LC斑点清晰，分离度良好，方法耐用性佳；H PLC方法灵敏度高、重复性好、精密度高，山柰酚的线性范围为12．01～384．56μg · mL －1（r＝0．9999），平均回收率为97．60％，RSD为2．07％（n＝9）。结论建立的方法简便，结果准确可靠，可用于柿叶中黄酮与三萜类成分的定性定量分析。
목적：완선시협적정성정량방법，편우저일약용자원적질량공제。방법채용박층층석색보법（T LC ）대시협중곡피소、산내분、제돈과산화웅과산진행정성감별；채용고효액상색보법（H PLC ）대불동래원시협중적산내분진행정량분석。색보주위Wondasil C18‐WR색보주（250 mm ×4．6 mm ，5μm）；류동상위갑순‐2 mL · L －1린산용액（52∶48），류속위1．0 mL · min－1，검측파장위360 nm ，이론판수안산내분봉계산응불저우8000。결과 T LC반점청석，분리도량호，방법내용성가；H PLC방법령민도고、중복성호、정밀도고，산내분적선성범위위12．01～384．56μg · mL －1（r＝0．9999），평균회수솔위97．60％，RSD위2．07％（n＝9）。결론건립적방법간편，결과준학가고，가용우시협중황동여삼첩류성분적정성정량분석。
Objective To optimize the qualitative and quantitative methods for leaves of Diospyros kaki Thunb .Methods Thin layer chromatography (TLC) was performed for identifying kaempferol ,quercetin ,oleanolic acid and ursolic acid .Kaempferol was de‐termined by HPLC .The chromatographic column was Wondasil C18‐WR(250 mm × 4 .6 mm ,5 μm) ,methanol‐2 mL · L -1 phos‐phoric acid solution (52∶48) as the mobile phase ,the flow rate was 1 .0 mL · min-1 ,and the detection wavelength was 360 nm . The number of theoretical plates for kaempferol was not less than 8 000 .Results The TLC spots were clear and can be well sepa‐rated ,indicating that the method had good ruggedness .The HPLC method was simple and specific .The calibration curve showed good linear relationship in the range of 12 .01‐384 .56 μg · mL -1 (r = 0 .999 9) .The average recovery was 97 .6% and RSD was 2 .07 % (n=9) .Conclusion The developed qualitative and quantitative methods were simple ,accurate and reliable ,and can be used for the quality control of leaves of Diospyros kaki Thunb .