CAJ | 학술논문

为深入开发提取碱溶性多糖，采用响应面法（RSM ）即碱提醇沉法对龙胆多糖进行提取，研究提取次数、提取温度、提取时间、液料比、碱浓度等因素对多糖提取率的影响。结果表明：在单因素试验基础上，结合响应面分析确定了提取碱溶性龙胆多糖的最适工艺条件为，提取温度74℃，料液比1∶25，提取时间1．8h，提取次数为1次，碱浓度为9％。模型预测的碱溶性龙胆多糖的提取率为4．4％，在此最佳提取参数下，碱溶性龙胆多糖的试验提取率为（4．32±0．124）％，与预期值相符。
위심입개발제취감용성다당，채용향응면법（RSM ）즉감제순침법대룡담다당진행제취，연구제취차수、제취온도、제취시간、액료비、감농도등인소대다당제취솔적영향。결과표명：재단인소시험기출상，결합향응면분석학정료제취감용성룡담다당적최괄공예조건위，제취온도74℃，료액비1∶25，제취시간1．8h，제취차수위1차，감농도위9％。모형예측적감용성룡담다당적제취솔위4．4％，재차최가제취삼수하，감용성룡담다당적시험제취솔위（4．32±0．124）％，여예기치상부。
For the further development of the alkali‐soluble polysaccharide extraction ,response surface method‐ology (RSM) was used to optimize the extraction conditions of alkaline solubility polysaccharides from the Gentiana scabra Bunge .The method of alkali extraction and alcohol sink was used to extract polysaccharides . The effect of extraction times ,extraction temperature ,extraction time ,ratio of water to raw material ,and NaOH concentration on the yield of Gentiana scabra Bunge polysaccharides were investigated .The results showed that combining single factor test and response surface methodology ,the optimum extraction conditions were chosen as follows:extraction temperature was 74℃ ,ratio of water to raw material was 1∶25 ,extraction time was 1 .8 h ,one time extraction and the NaOH concentration was 9% .Under the optimize conditions ,the GSP predicted value of yield was 4 .4% ,and the GSP experimental yield was 4 .32 ± 0 .124% ,the results were reasonable with expectation .