河南科技
河南科技
하남과기
Journal of Henan Science and Technology
2015年
10期
133-135
,共3页
纤维素降解菌%微生物群落%PCR-DGGE
纖維素降解菌%微生物群落%PCR-DGGE
섬유소강해균%미생물군락%PCR-DGGE
Cellulasedegradation%Microbial community%PCR-DGGE
采用兼性厌氧法筛选出一高效降解纤维素的复合微生物菌群,提取不同培养时间条件下降解菌体系的总DNA,并以其为模板采用PCR-DGGE技术研究了在纤维素降解过程中微生物群落的组成,结果表明,随着培养时间的不同,微生物的群落结构表现出差异.
採用兼性厭氧法篩選齣一高效降解纖維素的複閤微生物菌群,提取不同培養時間條件下降解菌體繫的總DNA,併以其為模闆採用PCR-DGGE技術研究瞭在纖維素降解過程中微生物群落的組成,結果錶明,隨著培養時間的不同,微生物的群落結構錶現齣差異.
채용겸성염양법사선출일고효강해섬유소적복합미생물균군,제취불동배양시간조건하강해균체계적총DNA,병이기위모판채용PCR-DGGE기술연구료재섬유소강해과정중미생물군락적조성,결과표명,수착배양시간적불동,미생물적군락결구표현출차이.
A highly effective complex microbial community in cellulose degradation was selected and obtained through afacultative anaerobic. The bacterial DNA was extracted under different cultivation time conditions, and the composition of microbial community in cellulose degradation was studied by PCR-DGGE technology based on the above module.The results showed that there were different structures of microbial community with changing cultivation time.