CAJ | 학술논문

为了探讨八眉猪 DRA 基因的遗传特性及其在抗病育种和猪-人异种器官移植中的应用前景，采用克隆测序的方法对该基因编码区进行多态性分析，并利用生物信息学方法预测该基因编码氨基酸的理化特性和结构功能，同时对该基因进行同源性分析。结果显示，八眉猪 DRA 基因编码252个氨基酸，编码区有5个核苷酸和2个氨基酸变异位点。DRA 基因编码产物是一种不稳定的不可溶蛋白，二级结构为混合型，无规则卷曲所占比例最高。蛋白质功能预测结果显示，DRA 基因免疫应答几率最高（4．405）。同源性分析发现，八眉猪 DRA 氨基酸与其他猪种的相似度在98％以上，与人的相似度达到83％，在 CD4分子结合部位与人类相应的 DRA 仅存在1个氨基酸差异，具有在异种移植排斥反应中利用的潜力。研究结果为八眉猪的抗病分子育种和猪-人异种器官移植提供理论依据。
위료탐토팔미저 DRA 기인적유전특성급기재항병육충화저-인이충기관이식중적응용전경，채용극륭측서적방법대해기인편마구진행다태성분석，병이용생물신식학방법예측해기인편마안기산적이화특성화결구공능，동시대해기인진행동원성분석。결과현시，팔미저 DRA 기인편마252개안기산，편마구유5개핵감산화2개안기산변이위점。DRA 기인편마산물시일충불은정적불가용단백，이급결구위혼합형，무규칙권곡소점비례최고。단백질공능예측결과현시，DRA 기인면역응답궤솔최고（4．405）。동원성분석발현，팔미저 DRA 안기산여기타저충적상사도재98％이상，여인적상사도체도83％，재 CD4분자결합부위여인류상응적 DRA 부존재1개안기산차이，구유재이충이식배척반응중이용적잠력。연구결과위팔미저적항병분자육충화저-인이충기관이식제공이론의거。
The study aimed to elucidate the genetic characters of DRA gene in Bamei pigs and the potential in disease-resistance breeding and pigs to humans xenotransplantation,polymorphism of DRA gene coding regions in Bamei pigs were analyzed by DNA sequencing analysis,and further physicochemical characteristics,protein struc-tures and functions were predicted using the bioinformatics tools and the homologies of amino acid sequences be-tween SLA and HLA were compared.The results showed that DRA gene encoded 252 amino acids,there were only 5 nucleotide and 2 amino acid substitutions in DRA gene coding regions.Encoding product of DRA gene was a kind of instable and insoluble protein,the putative secondary structure of DRA was mixed,and the percent of random coil was highest.The putative result of DRA protein function showed that the odds of immune response were highest (4.405).The homologies of DRA amino acid sequences between Bamei and other pig breeds were more than 98%, and the homologies between Bamei and human were 83%,the position which bind to human CD4 showed only one difference with HLA-DRA,which had potential using in xenograft rejection.These results would provide a basis for the study of molecular breeding in disease resistance and xenotransplantation.