华北农学报
華北農學報
화북농학보
Acta Agriculturae Boreali-Sinica
2015年
4期
59-65
,共7页
宋培玲%Malgorzata Jedryczka%郝丽芬%皇甫海燕%李子钦%包玉英
宋培玲%Malgorzata Jedryczka%郝麗芬%皇甫海燕%李子欽%包玉英
송배령%Malgorzata Jedryczka%학려분%황보해연%리자흠%포옥영
油菜%黑胫病%抗性评价%抗病基因推导
油菜%黑脛病%抗性評價%抗病基因推導
유채%흑경병%항성평개%항병기인추도
Oilseed rape%Phoma stem canker%Resistance evaluation%Resistance gene postulation
为掌握中国油菜品种的黑胫病抗性水平,为抗病育种及品种合理布局提供理论依据。在室内采用子叶穿刺接种法,对35个油菜品种开展了黑胫病的抗性评价及抗病基因推导研究。供试品种中没有发现对病原菌 Lepto-sphaeria maculans 和 Leptosphaeria biglobosa 表现高抗或免疫的品种,只有3个品种可以兼抗 Leptosphaeria maculans 和Leptosphaeria biglobosa,但均表现低抗。供试的35个品种对12个菌株共产生了17种反应型,其中12个品种的反应型与鉴别寄主的完全相同,18个品种的反应型与鉴别寄主的非常相近,5个品种的反应型不同于任何鉴别寄主。抗病基因推导,已知的12个黑胫病抗性基因 Rlm1~9,Lep R1~3多数以基因组合的形式分布在我国的30个品种中,这些主栽品种可能含有已知的或新的抗病基因。说明现有的油菜种质资源中可能蕴藏着对改良黑胫病抗性具有潜在应用价值的基因资源。
為掌握中國油菜品種的黑脛病抗性水平,為抗病育種及品種閤理佈跼提供理論依據。在室內採用子葉穿刺接種法,對35箇油菜品種開展瞭黑脛病的抗性評價及抗病基因推導研究。供試品種中沒有髮現對病原菌 Lepto-sphaeria maculans 和 Leptosphaeria biglobosa 錶現高抗或免疫的品種,隻有3箇品種可以兼抗 Leptosphaeria maculans 和Leptosphaeria biglobosa,但均錶現低抗。供試的35箇品種對12箇菌株共產生瞭17種反應型,其中12箇品種的反應型與鑒彆寄主的完全相同,18箇品種的反應型與鑒彆寄主的非常相近,5箇品種的反應型不同于任何鑒彆寄主。抗病基因推導,已知的12箇黑脛病抗性基因 Rlm1~9,Lep R1~3多數以基因組閤的形式分佈在我國的30箇品種中,這些主栽品種可能含有已知的或新的抗病基因。說明現有的油菜種質資源中可能蘊藏著對改良黑脛病抗性具有潛在應用價值的基因資源。
위장악중국유채품충적흑경병항성수평,위항병육충급품충합리포국제공이론의거。재실내채용자협천자접충법,대35개유채품충개전료흑경병적항성평개급항병기인추도연구。공시품충중몰유발현대병원균 Lepto-sphaeria maculans 화 Leptosphaeria biglobosa 표현고항혹면역적품충,지유3개품충가이겸항 Leptosphaeria maculans 화Leptosphaeria biglobosa,단균표현저항。공시적35개품충대12개균주공산생료17충반응형,기중12개품충적반응형여감별기주적완전상동,18개품충적반응형여감별기주적비상상근,5개품충적반응형불동우임하감별기주。항병기인추도,이지적12개흑경병항성기인 Rlm1~9,Lep R1~3다수이기인조합적형식분포재아국적30개품충중,저사주재품충가능함유이지적혹신적항병기인。설명현유적유채충질자원중가능온장착대개량흑경병항성구유잠재응용개치적기인자원。
In order to master the resistance level,we provide a theoretical basis for blackleg resistance breeding and varieties rational distribution.35 cultivars were tested to identify and postulate their resistances to Phoma stem canker using the cotyledons puncture method.No high resistance or immune varieties to Leptosphaeria maculans and Leptosphaeria biglobosa were found in the 35 oilseed rape cultivars.Only three varieties showed low resistance to both Leptosphaeria maculans and Leptosphaeria biglobosa.35 varieties produced 1 7 reaction types to 1 2 strains,total-ly.Among which,the reaction type of 1 2 varieties were same with the differential hosts,the reaction type of 1 8 varie-ties were very similar to the differential hosts,the reaction type of 5 varieties were unlike any differential hosts. Based on the genetic postulate method,we postulated that the twelve genes known blackleg resistance (Rlm1-9, LepR1-3)were mainly in form of combinations in thirty cultivars,these main oilseed rape cultivars may carry the known or other new resistance genes.So,some genetic resources in our rape germplasm could be used potentially to modify the resistance to Phoma stem canker.