CAJ | 학술논문

为获得具有高效、广谱拮抗作用的生防细菌。从实验室及葡萄、黄瓜、西红柿的根和根际土壤中培养得到282株细菌，以13种常见植物病原真菌为靶标菌，通过平板对峙法和琼脂扩散法测定了分离细菌的发酵液和发酵滤液对靶标菌的抑制效果。结果表明：共有9株分离细菌具有较好的抑制作用，其中4株拮抗细菌的发酵液对所有13株靶标病原菌均有很好的抑制作用，表现出较为广谱的抑菌作用。其中 k8j 菌株的抑菌效果与其它拮抗菌株达到了显著性水平，其对玉米大斑病菌（Setosphaeria turcica）的抑制率最高，达到了92．02％。9株拮抗细菌的发酵滤液对不同的靶标病原菌也表现出一定的抑制作用，其中 HMGR‐2菌株对玉米大斑病菌的抑制效果最好，抑菌圈直径达到了4．15 cm ；菌株 k8j 的发酵滤液对11株病原菌均具有很好的抑菌效果（除水稻稻瘟病菌和番茄灰霉病菌外），并与其它拮抗菌株比较达到了显著性水平，说明该菌株的代谢产物也具有较为广谱的抑菌效果。
위획득구유고효、엄보길항작용적생방세균。종실험실급포도、황과、서홍시적근화근제토양중배양득도282주세균，이13충상견식물병원진균위파표균，통과평판대치법화경지확산법측정료분리세균적발효액화발효려액대파표균적억제효과。결과표명：공유9주분리세균구유교호적억제작용，기중4주길항세균적발효액대소유13주파표병원균균유흔호적억제작용，표현출교위엄보적억균작용。기중 k8j 균주적억균효과여기타길항균주체도료현저성수평，기대옥미대반병균（Setosphaeria turcica）적억제솔최고，체도료92．02％。9주길항세균적발효려액대불동적파표병원균야표현출일정적억제작용，기중 HMGR‐2균주대옥미대반병균적억제효과최호，억균권직경체도료4．15 cm ；균주 k8j 적발효려액대11주병원균균구유흔호적억균효과（제수도도온병균화번가회매병균외），병여기타길항균주비교체도료현저성수평，설명해균주적대사산물야구유교위엄보적억균효과。
In order to obtain bio‐control bacteria with highly effective and broad spectrum antagonism effect a‐gainst plant pathogenic fungi ,282 isolates were obtained by the method of dilution coated tablet and streak plate from lab and roots and rhizosphere soil samples of grapes ,tomatoes and cucumbers .With 13 kinds of common plant pathogenic fungi as indicators ,inhibitory effect of fermentation liquid and fermentation filtrate a‐gainst the indicators were tested by the methods of tablet confrontation and agar disk diffusion .The results showed that 9 bacterial strains had efficient and stable inhibitory activity ,and 4 strains of them fermentation liquid had efficient inhibitory activity against all the indicators ,showing broad antimicrobial spectrum against pathogenic fungi .Among them ,the inhibition effect of k8j was significantly higher than other isolates ,and it had the highest inhibition effect against Setosphaeria turcica ,with the inhibition rate was 92 .02% .9 bacterial strains fermentation filtrate also showed some inhibitory effect .HMGR‐2 had the best inhibition effect against Setosphaeria turcica with the inhibition zone diameters was 4 .15 cm .Fermentation filtrate of k8j had good in‐hibition effect against 11 indicators (except for Pyricularia grisea and Botrytis cinerea) ,and compared with other strains ,its inhibitory action reached the significant level ,and it showed that metabolites also had broad spectrum of antibacterial effect .