CAJ | 학술논문

目的：观察丁酸钠联合顺铂对卵巢透明细胞癌ES-2细胞活性及增殖的影响。方法将对数生长期的卵巢透明细胞癌ES-2细胞随机分为观察组和对照组。观察组分别加入1、2、4、6 mmol／L的丁酸钠和5μg／mL顺铂，对照组分别加入与观察组相同浓度的丁酸钠。采用MMT法检测两组细胞活性，Western blotting 法检测两组凋亡蛋白（ c-PAPP、c-Caspase-3）和增殖蛋白（ ERK、p-ERK）相对表达量。结果观察组加入1、2、4、6 mmol／L丁酸钠和顺铂细胞活性分别为51％±3％、39％±2％、22％±2％、11％±1％，多组间及组内两两比较，P均＜0．05；对照组加入1、2、4、6 mmol／L丁酸钠细胞活性分别为88％±4％、76％±3％、69％±2％、54％±2％，多组间及组内两两比较，P均＜0．05；两组加入相同浓度丁酸钠细胞活性比较，P均＜0．05。随着丁酸钠浓度增加，两组凋亡蛋白c-PAPP、c-Caspase-3相对表达量逐渐升高，增殖蛋白ERK、p-ERK相对表达量逐渐降低。与对照组同浓度丁酸钠比较，观察组1、2、4、6 mmol／L丁酸钠和顺铂c-PAPP、c-Caspase-3蛋白表达均升高，1、6 mmol／L丁酸钠和顺铂ERK、p-ERK蛋白表达均降低；两组比较，P均＜0．05。结论丁酸钠联合顺铂可降低卵巢透明细胞癌ES-2细胞活性，抑制其增殖、促进其凋亡；丁酸钠浓度越高，上述作用越明显。
목적：관찰정산납연합순박대란소투명세포암ES-2세포활성급증식적영향。방법장대수생장기적란소투명세포암ES-2세포수궤분위관찰조화대조조。관찰조분별가입1、2、4、6 mmol／L적정산납화5μg／mL순박，대조조분별가입여관찰조상동농도적정산납。채용MMT법검측량조세포활성，Western blotting 법검측량조조망단백（ c-PAPP、c-Caspase-3）화증식단백（ ERK、p-ERK）상대표체량。결과관찰조가입1、2、4、6 mmol／L정산납화순박세포활성분별위51％±3％、39％±2％、22％±2％、11％±1％，다조간급조내량량비교，P균＜0．05；대조조가입1、2、4、6 mmol／L정산납세포활성분별위88％±4％、76％±3％、69％±2％、54％±2％，다조간급조내량량비교，P균＜0．05；량조가입상동농도정산납세포활성비교，P균＜0．05。수착정산납농도증가，량조조망단백c-PAPP、c-Caspase-3상대표체량축점승고，증식단백ERK、p-ERK상대표체량축점강저。여대조조동농도정산납비교，관찰조1、2、4、6 mmol／L정산납화순박c-PAPP、c-Caspase-3단백표체균승고，1、6 mmol／L정산납화순박ERK、p-ERK단백표체균강저；량조비교，P균＜0．05。결론정산납연합순박가강저란소투명세포암ES-2세포활성，억제기증식、촉진기조망；정산납농도월고，상술작용월명현。
Objective To observe the effect of sodium butyrate combined with cisplatin on cell viability and prolifera -tion of ovarian clear cell carcinoma ES-2 cells.Methods The ovarian clear cell carcinoma ES-2 cells in the logarithmic phase were randomly divided into the observation groups and control groups .The observation groups were added 1, 2, 4 and 6 mmol/L sodium butyrate (NaB) and 5 μg/mL cisplatin.The control groups were added the same concentrations of NaB.The cell activities were detected by MMT , and the relative expression levels of c-PAPP, c-Caspase-3, ERK and p-ERK were detected by Western blotting .Results The cell viabilities of the observation groups which were treated with 1, 2, 4, 6 mmol/L NaB and 5 μg/mL cisplatin were 51%±3%, 39%±2%, 22%±2% and 11%±1%, respectively;and significant difference was found between groups (all P<0.05).The cell viabilities of the control groups were 88%± 4%, 76%±3%, 69%±2%and 54%±2%, respectively;and significant difference was found between groups ( all P<0.05).Meanwhile, significant difference was found in the cell viability between the two groups which were added with the same concentrations of NaB (all P<0.05).With the increased concentrations of NaB , the relative expression levels of c-PAPP and c-Caspase-3 in the two kinds of groups were gradually increased , and the levels of ERK and p-ERK were signifi-cantly decreased .Compared with the control groups , the relative expression levels of c-PAPP and c-Caspase-3 in the obser-vation groups which were added 1, 2, 4 and 6 mmol/L NaB and 5 μg/mL cisplatin were all increased and the levels of ERK and P-ERK were decreased in the observation groups which were added 1 and 6 mmol/L NaB and 5μg/mL cisplatin. Significant difference was found between the two kinds of groups (all P<0.05).Conclusion NaB combined with cispla-tin may decrease the cell viability of ovarian clear cell carcinoma ES-2 cells, inhibit the proliferation and promote the apop-tosis.The effect can be stronger with the higher concentrations of NaB .