CAJ | 학술논문

目的：对FQ-PCR检测BALF中结核杆菌-DNA对肺结核诊断的应用进行研究。方法：随机选取2013年12月-2014年11月期间本院收治的肺结核患者110例作为本次研究的对象，所有患者实施纤支镜检查、活检、刷检，BALF中结核杆菌-DNA行FQ-PCR检测，对检查结果进行分析研究。结果：在本次研究中，确诊为肺结核的患者有40.00%（44/110）；FQ-PC检测的敏感性明显高于常规涂片镜检，差异有统计学意义（P<0.05）。结论：在肺结核的诊断中，FQ-PCR检测的敏感度高，操作简单方便，检测速度较快，是BALF中结核杆菌-DNA检查的重要方法，在肺结核的诊断中有重要的应用价值。
목적：대FQ-PCR검측BALF중결핵간균-DNA대폐결핵진단적응용진행연구。방법：수궤선취2013년12월-2014년11월기간본원수치적폐결핵환자110례작위본차연구적대상，소유환자실시섬지경검사、활검、쇄검，BALF중결핵간균-DNA행FQ-PCR검측，대검사결과진행분석연구。결과：재본차연구중，학진위폐결핵적환자유40.00%（44/110）；FQ-PC검측적민감성명현고우상규도편경검，차이유통계학의의（P<0.05）。결론：재폐결핵적진단중，FQ-PCR검측적민감도고，조작간단방편，검측속도교쾌，시BALF중결핵간균-DNA검사적중요방법，재폐결핵적진단중유중요적응용개치。
Objective:To study the application of mycobacterium tuberculosis-DNA in BALF by FQ-PCR for the diagnosis of pulmonary.Method:110 cases with tuberculosis in our hospital were randomly selected from December 2013 to November 2014 as the objects of the study, all patients were given the fiberoptic bronchoscopy, biopsy, brush biopsy, mycobacterium tuberculosis DNA in bronchoalveolar lavage fluid (BALF) was detected by FQ-PCR,the examination results were analyzed.Result: In this study,44 cases of pulmonary tuberculosis, accounted for the proportion to 40.00%(44/110);the sensitivity of FQ-PC detection was significantly higher than that of conventional smear microscopy,the difference was statistically significant(P<0.05).Conclusion:In the diagnosis of pulmonary tuberculosis,the FQ-PCR detection has high sensitivity, simple operation and the detection speed faster,it is an important examination method of mycobacterium tuberculosis DNA in BALF and has important application value.