CAJ | 학술논문

为了创新蓖麻的种质资源，以通篦5号为材料，用0，0．02，0．03，0．04，0．05，0．06 mol ／L 的 EMS 溶液处理去壳的蓖麻种子，处理时间分别设定4，8，12 h，研究不同处理浓度和时间组合对蓖麻种子发芽率和根长的影响。结果表明：用0．04 mol ／L 的 EMS 溶液处理4 h，蓖麻种子的相对发芽率为49％，为半致死剂量处理组合，平均主根长10．45 mm。随着处理浓度的增加和处理时间的延长，蓖麻种子的相对发芽率和平均根长均表现下降趋势，处理浓度到0．06 mol ／L 时，蓖麻种子完全不能萌发；对10个突变体 ISSR 分子标记分析发现，M2发生了分子水平的变异，引物 P91扩增出的多态性位点达13个。
위료창신비마적충질자원，이통비5호위재료，용0，0．02，0．03，0．04，0．05，0．06 mol ／L 적 EMS 용액처리거각적비마충자，처리시간분별설정4，8，12 h，연구불동처리농도화시간조합대비마충자발아솔화근장적영향。결과표명：용0．04 mol ／L 적 EMS 용액처리4 h，비마충자적상대발아솔위49％，위반치사제량처리조합，평균주근장10．45 mm。수착처리농도적증가화처리시간적연장，비마충자적상대발아솔화평균근장균표현하강추세，처리농도도0．06 mol ／L 시，비마충자완전불능맹발；대10개돌변체 ISSR 분자표기분석발현，M2발생료분자수평적변이，인물 P91확증출적다태성위점체13개。
In order to innovate castor germplasm,hulled castor seeds were treated with EMS at 6 levels (0, 0.02,0.03,0.04,0.05,0.06 mol /L)and different time(4,8,1 2 h),The results showed that lethal concentration 50 was at 0.04 mol /L and 4 h,the average taproot length was 1 0.45 mm,and the concentration of EMS reached to 0.06 mol /L,the seeds could not germinate completely.With the increasing of treatment time and concentration,cas-tor seed germination rate and average relative root length showed a downward trend;ISSR molecular marker analysis showed that DNA variation existed in M2 by EMS.The primer P91 detected 1 3 variation loci.