CAJ | 학술논문

为了给筛选萼脊兰内参基因和研究 TUB 基因的生理功能提供研究基础，对萼脊兰 TUB 基因片段进行克隆与序列分析。采用 CTAB 法提取萼脊兰盛花期花瓣总 RNA，并运用 RT-PCR 方法克隆萼脊兰 TUB 基因序列，长度为1055 bp，编码351个氨基酸残基，氨基酸序列中存在微管蛋白的保守区域 GGGTGSG。序列分析结果表明：与其他已登录物种的 TUB 基因相比，其核苷酸序列的同源性达75％～96％，与朵丽蝶兰（JN185665．1）同源性高达96％，氨基酸序列的同源性也在96％以上，且与单子叶植物的同源序列表现出较高的相似性。在 GenBank 注册，登录号为KP686397。
위료급사선악척란내삼기인화연구 TUB 기인적생리공능제공연구기출，대악척란 TUB 기인편단진행극륭여서렬분석。채용 CTAB 법제취악척란성화기화판총 RNA，병운용 RT-PCR 방법극륭악척란 TUB 기인서렬，장도위1055 bp，편마351개안기산잔기，안기산서렬중존재미관단백적보수구역 GGGTGSG。서렬분석결과표명：여기타이등록물충적 TUB 기인상비，기핵감산서렬적동원성체75％～96％，여타려접란（JN185665．1）동원성고체96％，안기산서렬적동원성야재96％이상，차여단자협식물적동원서렬표현출교고적상사성。재 GenBank 주책，등록호위KP686397。
TUB of Sedirea japonica was cloned and sequence analyzed,and the result would provide an impor-tant basis for selecting a reference gene and studying the function of TUB.The total RNA of Sedirea japonica scapes was extracted by CTAB method and the partial sequence of Sedirea japonica TUB was cloned by RT-PCR,which was 1 055 bp long and encoded 351 amino acids residues.Its amino acid sequence contained a conservative region GGGTGSG.The sequencing result revealed that the TUB gene fragment from Sedirea japonica homology comparison with other plants TUB gene sequences in the GenBank shows that they share 75%-96% nucleotide sequence ho-mology,96% nucleotide sequence homology with Doritaenopsis hybrid TUB,the amino acid sequence homology is a-bove 96% and amino acid sequence homology high similarity with the monocotyledon.The cloned genes were TUB gene fragment,which were registered them into GenBank(respective accession number:KP686397).