CAJ | 학술논문

为了利用基因工程手段改变谷子中氨基酸组成和含量，进而改善谷子营养品质，以谷子栽培品种豫谷1号幼嫩、无病斑的叶片基因组 DNA 为模板，根据已报道的玉米天冬氨酸转氨酶基因序列，设计1对特异性引物扩增克隆谷子天冬氨酸转氨酶基因部分序列。结果表明，PCR 扩增获得约750 bp 的目标片段，对该片段进行克隆测序得到一条742 bp 的片段，经公共数据库同源搜索发现该序列含有天冬氨酸转氨酶超基因家族特有的保守域，证明所克隆片段为天冬氨酸转氨酶基因。对该基因进行系统进化树分析发现，部分单子叶植物和双子叶植物分别聚类，但是谷子与玉米、甘蔗3个 C4作物并没有聚为一类。为进一步揭示谷子天冬氨酸转氨酶的功能奠定了基础。
위료이용기인공정수단개변곡자중안기산조성화함량，진이개선곡자영양품질，이곡자재배품충예곡1호유눈、무병반적협편기인조 DNA 위모판，근거이보도적옥미천동안산전안매기인서렬，설계1대특이성인물확증극륭곡자천동안산전안매기인부분서렬。결과표명，PCR 확증획득약750 bp 적목표편단，대해편단진행극륭측서득도일조742 bp 적편단，경공공수거고동원수색발현해서렬함유천동안산전안매초기인가족특유적보수역，증명소극륭편단위천동안산전안매기인。대해기인진행계통진화수분석발현，부분단자협식물화쌍자협식물분별취류，단시곡자여옥미、감자3개 C4작물병몰유취위일류。위진일보게시곡자천동안산전안매적공능전정료기출。
The genomic DNA extracted from young,no-lesion leaves of foxtail millet cultivar Yugu 1 was as template,and a pair of specific primers was designed according to the reported corn aspartate aminotransferase gene sequence to clone aspartate transaminase partial gene sequence of foxtail millet,so as to provide foundation for alte-ring composition and content of amino acids by genetic engineering,further altering nutritional quality of foxtail mil-let.The results showed that:PCR amplification obtained a 750 bp target fragment.After cloning and sequencing,a 742 bp fragment was obtained.After Blast search through public database,a specific conserved domain belonging to aspartate aminotransferase supergene family was found in cloned sequence,which proved that the cloned fragment was aspartate aminotransferase gene.Then a phylogenetic tree based on the gene sequences was constructed,which indicated that part of monocots and dicots could cluster respectively,but foxtail millet,maize and sugar cane,three C4 plants,could not cluster together.This study provided foundation for further revealing the function of aspartate aminotransferase gene of foxtail millet.