CAJ | 학술논문

目的：探讨食管鳞状细胞癌组织P16基因启动子区CpG单位的甲基化与其发病的关系。方法收集食管鳞状细胞癌标本35份（观察组）和正常食管组织46份（对照组），采用MassARRAY甲基化DNA定量分析技术检测两组P16基因启动子区19个CpG单位的甲基化状态，秩和检验比较两组CpG单位的甲基化率。结果观察组P16基因启动子区CpG单位总甲基化率为9．06％±7．11％，对照组为8．48％±6．34％；两组比较，P＞0．05。观察组P16基因启动子区CpG＿11-12位点甲基化率为11．34％±8．47％，对照组为7．25％±5．60％；两组比较，P＜0．05；两组其余18个CpG单位甲基化率比较，P均＞0．05。结论 P16基因启动子区CpG＿11-12位点的甲基化可能与食管鳞状细胞癌的发病有关。
목적：탐토식관린상세포암조직P16기인계동자구CpG단위적갑기화여기발병적관계。방법수집식관린상세포암표본35빈（관찰조）화정상식관조직46빈（대조조），채용MassARRAY갑기화DNA정량분석기술검측량조P16기인계동자구19개CpG단위적갑기화상태，질화검험비교량조CpG단위적갑기화솔。결과관찰조P16기인계동자구CpG단위총갑기화솔위9．06％±7．11％，대조조위8．48％±6．34％；량조비교，P＞0．05。관찰조P16기인계동자구CpG＿11-12위점갑기화솔위11．34％±8．47％，대조조위7．25％±5．60％；량조비교，P＜0．05；량조기여18개CpG단위갑기화솔비교，P균＞0．05。결론 P16기인계동자구CpG＿11-12위점적갑기화가능여식관린상세포암적발병유관。
Objective To detect the CpG unit methylation in the P 16 gene promoter region of esophageal squamous cell carcinoma tissues and the significance .Methods We collected 35 cases of esophageal squamous cell carcinoma sam-ples ( observation group ) and 46 cases of normal esophageal tissues ( control group ) .We used MassARRAY methylation DNA quantitative analysis technology to detect the methylation status of 19 CpG units of P16 gene promoter region , and we used Rank test to compare the CpG methylation rates of the two groups .Results The 19 CpG unit methylation rates in the P16 gene promoter region of the observation group and control group were 9.06%±7.11%and 8.48%±6.34%, respective-ly.No significant difference was found between the two groups , P>0.05.The methylation rate of P16 gene in ESCC of CpG unit 11-12 was 11.34%±8.47%in the observation group, and 7.25%±5.60%in the control group (P<0.05);and no significance was found in the methylation rate of other 18 units between the two groups ( all P>0.05 ) .Conclusion The methylation of CpG11-12 site in the P16 gene promoter region may be associated with the esophageal squamous cell carcinoma .