CAJ | 학술논문

为了进一步分离纯化抑菌蛋白和脂肽类物质，促进拮抗菌的开发和利用，选取2株对多种植物病原真菌具有良好拮抗作用的解淀粉芽孢杆菌，利用硫酸铵沉淀法粗提2株菌株的抑菌蛋白，酸沉淀法粗提脂态类抑菌物质，分别测定粗提物对多种植物病原真菌的抑制效果。初步检测2株菌株是否具有产纤维素酶、蛋白酶和嗜铁素活性。结果表明：BQD1菌株20％～30％的蛋白粗提液对玉米大斑病菌拮抗作用最强，抑菌直径达31．48 mm 。 TY 菌株30％～40％的蛋白粗提液对玉米大斑病菌抑菌直径最高达34．20 mm 。2株菌株脂态类粗提液对靶标病原菌株的拮抗作用均高于其发酵液，且均可产生纤维素酶、蛋白酶和嗜铁素。
위료진일보분리순화억균단백화지태류물질，촉진길항균적개발화이용，선취2주대다충식물병원진균구유량호길항작용적해정분아포간균，이용류산안침정법조제2주균주적억균단백，산침정법조제지태류억균물질，분별측정조제물대다충식물병원진균적억제효과。초보검측2주균주시부구유산섬유소매、단백매화기철소활성。결과표명：BQD1균주20％～30％적단백조제액대옥미대반병균길항작용최강，억균직경체31．48 mm 。 TY 균주30％～40％적단백조제액대옥미대반병균억균직경최고체34．20 mm 。2주균주지태류조제액대파표병원균주적길항작용균고우기발효액，차균가산생섬유소매、단백매화기철소。
In order to isolate and purify the antifungal proteins and lipopeptides products ,and promote the devel‐opment and utilization of antagonistic bacteria ,two strains of Bacillus amylolique f aciens which showed obvi‐ous antifungal activity against plant pathogens were selected .Antifungal proteins of two strains were prelimina‐ry separated ,using the methods of ammonium sulfate precipitation ,and tested on plant pathogens .Antifungal activity of lipopeptides products which extracted with methanol from hydrochloric acid precipitate were tested plant pathogens .Two strains were also tested wether could produce cellulose ,protease and siderophore .The re‐sults showed that the strongest antifungal proteins inhibition activity of strain BQD1 was under the ammonium sulfate condition of 20% ~ 30% ,its diameter of inhibition zone to Setosphaeria turcica was 31 .48 mm .Anti‐fungal proteins inhibition activity of strain TY was under the ammonium sulfate condition of 30% ~ 40% ,its diameter of inhibition zone to Setosphaeria turcica was 34 .20 mm .Lipopeptides products of two strains had stronger antagonism effect on target plant pathogens than its ferfentation .two strains could both produce cellu‐lose ,protease and siderophore .