CAJ | 학술논문

[目的]探讨双歧杆菌对实验性末端回肠炎肠组织 T L R2、T L R4的表达影响及干预机制。[方法]将90只健康雄性的SD大鼠随机分为正常组、模型组以及双歧杆菌干预组（每组30只）。模型组、双歧杆菌干预组均采用回肠末端‐盲肠侧侧吻合术建造实验性末端回肠炎动物模型，术后当日双歧杆菌干预组大鼠给予浓度为108 cf u／ml双歧杆菌悬液1 ml进行灌胃处理，正常组和模型组每日灌胃1 ml的10％脱脂乳。分别在术后第2周和第8周时处死大鼠，收集末端回肠组织，肉眼和显微镜下观察末端回肠组织的病理学改变、免疫组化及Western blot法检测肠上皮细胞中TLR2、TLR4的表达情况。[结果]第2周时，模型组与双歧杆菌干预组末端回肠出现急性炎症反应，与正常组比较，炎症评分均增加（P＜0.05，P＜0.01），正常组黏膜基本正常；第8周时，双歧杆菌干预组炎症评分较模型组明显降低（P＜0.01），双歧杆菌干预组组织基本正常，正常组无明显变化。第2周时，模型组、双歧杆菌干预组末端回肠黏膜肠上皮细胞组织中TLR2、TLR4的表达含量均高于正常组（P＜0.01，P＜0.05）；第8周时，模型组TLR2、TLR4表达强度比正常组高（P＜0.01），双歧杆菌干预组比2周时有所降低（P＜0.05），正常组无明显变化。[结论]双歧杆菌可通过抑制大鼠末端回肠组织中TLR2、TLR4表达下降从而达到一定的保护作用。
[목적]탐토쌍기간균대실험성말단회장염장조직 T L R2、T L R4적표체영향급간예궤제。[방법]장90지건강웅성적SD대서수궤분위정상조、모형조이급쌍기간균간예조（매조30지）。모형조、쌍기간균간예조균채용회장말단‐맹장측측문합술건조실험성말단회장염동물모형，술후당일쌍기간균간예조대서급여농도위108 cf u／ml쌍기간균현액1 ml진행관위처리，정상조화모형조매일관위1 ml적10％탈지유。분별재술후제2주화제8주시처사대서，수집말단회장조직，육안화현미경하관찰말단회장조직적병이학개변、면역조화급Western blot법검측장상피세포중TLR2、TLR4적표체정황。[결과]제2주시，모형조여쌍기간균간예조말단회장출현급성염증반응，여정상조비교，염증평분균증가（P＜0.05，P＜0.01），정상조점막기본정상；제8주시，쌍기간균간예조염증평분교모형조명현강저（P＜0.01），쌍기간균간예조조직기본정상，정상조무명현변화。제2주시，모형조、쌍기간균간예조말단회장점막장상피세포조직중TLR2、TLR4적표체함량균고우정상조（P＜0.01，P＜0.05）；제8주시，모형조TLR2、TLR4표체강도비정상조고（P＜0.01），쌍기간균간예조비2주시유소강저（P＜0.05），정상조무명현변화。[결론]쌍기간균가통과억제대서말단회장조직중TLR2、TLR4표체하강종이체도일정적보호작용。
[Objective]To investigate the expression of TLR2 and TLR4 and intervention mechanism in experimental terminal ileitis with bifidobacterium treatment. [Methods]Ninety healthy male SD rats were randomly divided into control group ,model group ,bifidobacterium intervention group. The model of termi‐nal ileitis was induced by ileum‐cecum side‐to‐side anastomosis in the model group and bifidobacterium in‐tervention group. The rats in bifidobacterium intervention group were given 1 mL bifidobacterium suspen‐sion of 108cfu/ml concentration postoperatively.The rats in control group and model group were daily giv‐en 1 ml of 10% skimmed milk. Euthanasia was performed 2 or 8 weeks postoperative ,and the terminal ile‐um tissues were collected. The pathological changes of the terminal ileum tissues were investigated by gross and microscopic observation ,and the expression of TLR2 and TLR4 in intestinal epithelial cells was detec‐ted. [Results]At 2 weeks ,the gross and microscopic observation showed acute inflammation in terminal ile‐um in model group and bifidobacterium intervention group.Inflammatory score of model group increased when comparing with control group(P<0.05 ,P<0.01) ,and the mucosa in control group was normal.At 8 weeks ,the inflammation score of bifidobacterium intervention group decreased significantly when compa‐ring with model group(P< 0.01).The tissues of bifidobacterium intervention group and control group were normal. The expression of TLR2 and TLR4 in the intestinal epithelium of model group and bifidobac‐terium intervention group were higher than control group (P<0.01 ,P<0.05). At 8 weeks ,the expression of TLR2 and TLR4 in model group were higher than control group(P<0.01) ,whereas they were reduced in bifidobacterium intervention group (P< 0.05). The control group had no significant changes. [Conclu‐sion]The expression of TLR2 and TLR4 increased in the process of experimental terminal ileitis ,and the expression intensity of TLR2 and TLR4 was related to inflammation level. The expression of TLR2 and TLR4 were decreased after intervention of bifidobacterium.