CAJ | 학술논문

目的探讨丙型肝炎病毒(HCV)核心抗原酶联免疫吸附技术(HCV-cAg ELISA)筛查献血员感染HCV的可行性.方法将183份抗-HCV阳性、6370份抗-HCV阴性(包括120份伴HBsAg阳性)献血员进行HCV RT-PCR和HCV-cAgELISA检测.结果抗-HCV阳性和抗-HCV阴性血清HCV RNA和HCV-cAg检测结果符合率分别为92.34％和99.97％.6370份抗-HCV阴性且不伴HBsAg阳性血清HCV-cAg阳性3份,其中1份HCV RNA阳性.HCV RNA拷贝数的上升与HCV-cAg阳性检出率上升呈正相关.结论 HCV-cAg ELISA敏感性与HCV RT-PCR类似,具有简便、快速、可靠优点,较适用于安全输血中献血者的血液筛检.
목적 탐토병형간염병독(HCV)핵심항원매련면역흡부기술(HCV-cAg ELISA)사사헌혈원감염HCV적가행성.방법 장183빈항-HCV양성、6370빈항-HCV음성(포괄120빈반HBsAg양성)헌혈원진행HCV RT-PCR화HCV-cAgELISA검측.결과 항-HCV양성화항-HCV음성혈청HCV RNA화HCV-cAg검측결과부합솔분별위92.34％화99.97％.6370빈항-HCV음성차불반HBsAg양성혈청HCV-cAg양성3빈,기중1빈HCV RNA양성.HCV RNA고패수적상승여HCV-cAg양성검출솔상승정정상관.결론 HCV-cAg ELISA민감성여HCV RT-PCR유사,구유간편、쾌속、가고우점,교괄용우안전수혈중헌혈자적혈액사검.
Objective To evaluate the feasibility to screen donor with hepatitis C virus (HCV) infection by HCV core antigen ELISA (HCV-cAgELISA).Methods 183 anti-HCV positive,6370 anti-HCV negative (including 120 specimens with HbsAg +) serum specimens by using HCV-cAgELISA and HCV RT-PCR methods.Results Comparing with HCV RT-PCR,the detection results of HCV-cAgELISA consistency were 92.34％ in anti-HCV positive serum specimens and 99.97％ in anti-HCV negative serum specimens,respectively.In 6370 anti-HCV negative specimens,1 serum sample was positive HCV RNA identification in 3 serum specimens which were positive for HCV-cAgELISA.HCV RNA copies and HCV-cAg positive rate was a positive correlation.Conclusion Sensitivity of HCVcAg ELISA is silllilar to HCV RT-PCR.HCVcAgELISA is a simple,fast,and reliable memod to screen donor with HCV infection in blood transfusion medicine.