中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
Chinese Journal of Experimental and Clinical Virology
2015年
4期
296-299
,共4页
周为民%谭心怡%李亚敏%谭文杰
週為民%譚心怡%李亞敏%譚文傑
주위민%담심이%리아민%담문걸
疱疹病毒科%细小病毒B19,人%肝功能试验%聚合酶链反应
皰疹病毒科%細小病毒B19,人%肝功能試驗%聚閤酶鏈反應
포진병독과%세소병독B19,인%간공능시험%취합매련반응
Herpes viridae%Parvovirus B19,human%Liver fumction tests%Polymerase chein reaction
目的 探讨人疱疹病毒(HHVs)和B19病毒感染在不明原因肝功能异常少儿人群中的病原学意义.方法 随机收集近年北京地区14岁以下少儿不明原因肝功能异常的患者血清样本62份,同时收集肝功能正常健康体检少儿血清123份作为对照,采用多重PCR法检测血清中的人疱疹病毒(HHV1-7型)的病毒核酸,用套式PCR法检测血清中的B19病毒核酸.结果 62份肝功能异常患者血清中HHVs与B19共计检出45例(72.58％),明显高于健康对照人群(52/123,42.28％);其中在不明原因肝功能异常少儿人群中检出率较高且明显高于正常人群(P值均＜0.05)的病毒主要为EBV(12.9％vs 3.3％)、CMV(33.9％vs 18.7％)、HHV6(19.4％ vs 5.7％)、B19 (41.9％vs26.8％).结论 人疱疹病毒(HHVs)和B19病毒感染与少儿人群中不明原因肝功能异常具有重要关联性,应引起重视.
目的 探討人皰疹病毒(HHVs)和B19病毒感染在不明原因肝功能異常少兒人群中的病原學意義.方法 隨機收集近年北京地區14歲以下少兒不明原因肝功能異常的患者血清樣本62份,同時收集肝功能正常健康體檢少兒血清123份作為對照,採用多重PCR法檢測血清中的人皰疹病毒(HHV1-7型)的病毒覈痠,用套式PCR法檢測血清中的B19病毒覈痠.結果 62份肝功能異常患者血清中HHVs與B19共計檢齣45例(72.58％),明顯高于健康對照人群(52/123,42.28％);其中在不明原因肝功能異常少兒人群中檢齣率較高且明顯高于正常人群(P值均＜0.05)的病毒主要為EBV(12.9％vs 3.3％)、CMV(33.9％vs 18.7％)、HHV6(19.4％ vs 5.7％)、B19 (41.9％vs26.8％).結論 人皰疹病毒(HHVs)和B19病毒感染與少兒人群中不明原因肝功能異常具有重要關聯性,應引起重視.
목적 탐토인포진병독(HHVs)화B19병독감염재불명원인간공능이상소인인군중적병원학의의.방법 수궤수집근년북경지구14세이하소인불명원인간공능이상적환자혈청양본62빈,동시수집간공능정상건강체검소인혈청123빈작위대조,채용다중PCR법검측혈청중적인포진병독(HHV1-7형)적병독핵산,용투식PCR법검측혈청중적B19병독핵산.결과 62빈간공능이상환자혈청중HHVs여B19공계검출45례(72.58％),명현고우건강대조인군(52/123,42.28％);기중재불명원인간공능이상소인인군중검출솔교고차명현고우정상인군(P치균＜0.05)적병독주요위EBV(12.9％vs 3.3％)、CMV(33.9％vs 18.7％)、HHV6(19.4％ vs 5.7％)、B19 (41.9％vs26.8％).결론 인포진병독(HHVs)화B19병독감염여소인인군중불명원인간공능이상구유중요관련성,응인기중시.
Objective To investigate the etiology significance of human herpes viruses (HHV1-7) and human Parvovirus B19 (B19) among children with abnormal liver function of unknown origin(ALUO).Methods Serum samples were randomly collected from 62 patients under 14 years old with ALUO in Beijing.And 123 serum samples from healthy children were collected as the control (normal cohort).The HHV1-7 and B19 infection were detected by multiple PCR and nested PCR,respectively.Results Total 45 samples from the patients group were detected as positive of HHV1-7 or B19 infection.The positive rate (72.58％,45/62) was significantly higher than the control group (52/123,42.28％).Furthermore,four viruses were dominant among pediatric patients with ALUO and significantly higher than the normal cohort,which were Epstein-Barr virus (EBV,12.9％ vs 3.3％),Cytomegalovirus (CMV,33.9％ vs 18.7％),human herpes viruses 6(HHV6,19.4％ vs 5.7％) and B19 (41.9％ vs 26.8％).Conclusions The significant correlation between the human herpes viruses (HHVs) and B19 infection and pediatric patients with ALUO need be considered and paid enough attention in clinic.
