中华普通外科杂志
中華普通外科雜誌
중화보통외과잡지
Chinese Journal of General Surgery
2015年
8期
635-638
,共4页
杨聚鹏%王大广%李亚刚%徐振国%所剑
楊聚鵬%王大廣%李亞剛%徐振國%所劍
양취붕%왕대엄%리아강%서진국%소검
胃肿瘤%增殖细胞核抗原%β连环素
胃腫瘤%增殖細胞覈抗原%β連環素
위종류%증식세포핵항원%β련배소
Stomach neoplasms%Proliferating cell nuclear antigen%beta-Catenin
目的 探讨β-连环蛋白(β-catenin)与增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)在胃癌组织中的表达,研究其与患者临床病理因素及预后的关系.方法 选取2000年1月至2006年12月间126例手术获得的胃癌组织和30例正常胃组织,构建组织芯片,应用免疫组化技术检测β-catenin和PCNA在胃癌及胃正常组织的表达,分析其与胃癌患者临床病理因素及预后的关系.结果 β-catenin和PCNA在胃癌组织中的阳性表达率分别为96.7％和99.2％,高于正常胃组织,差异有统计学意义(x2=41.082,P=0;x2=97.692,P=0);应用ROC曲线检测发现PCNA对胃癌和正常胃组织的鉴别优于β-catenin,前者的灵敏度、特异度及符合率分别为84.4％、87.5％及84.6％.β-catenin与淋巴结转移有关(x2=6.107.P=0.04),PCNA与病理分型有关(x2=9.71.P=0.02).β-catenin和PCNA在胃癌组织中的表达呈正相关(r=0.24,P=0.005).多因素分析显示胃癌患者的TNM分期及β-catenin和PCNA均阳性表达是影响患者生存的独立因素(P=0、P=0.04),而β-catenin、PCNA的单独阳性表达并不是影响胃癌患者生存的独立因素.结论 β-catenin和PCNA在胃癌组织中高表达.PCNA对胃癌和正常胃组织的鉴别优于β-catenin,联合检测两种蛋白对判断胃癌患者的预后具有一定的临床意义.
目的 探討β-連環蛋白(β-catenin)與增殖細胞覈抗原(proliferating cell nuclear antigen,PCNA)在胃癌組織中的錶達,研究其與患者臨床病理因素及預後的關繫.方法 選取2000年1月至2006年12月間126例手術穫得的胃癌組織和30例正常胃組織,構建組織芯片,應用免疫組化技術檢測β-catenin和PCNA在胃癌及胃正常組織的錶達,分析其與胃癌患者臨床病理因素及預後的關繫.結果 β-catenin和PCNA在胃癌組織中的暘性錶達率分彆為96.7％和99.2％,高于正常胃組織,差異有統計學意義(x2=41.082,P=0;x2=97.692,P=0);應用ROC麯線檢測髮現PCNA對胃癌和正常胃組織的鑒彆優于β-catenin,前者的靈敏度、特異度及符閤率分彆為84.4％、87.5％及84.6％.β-catenin與淋巴結轉移有關(x2=6.107.P=0.04),PCNA與病理分型有關(x2=9.71.P=0.02).β-catenin和PCNA在胃癌組織中的錶達呈正相關(r=0.24,P=0.005).多因素分析顯示胃癌患者的TNM分期及β-catenin和PCNA均暘性錶達是影響患者生存的獨立因素(P=0、P=0.04),而β-catenin、PCNA的單獨暘性錶達併不是影響胃癌患者生存的獨立因素.結論 β-catenin和PCNA在胃癌組織中高錶達.PCNA對胃癌和正常胃組織的鑒彆優于β-catenin,聯閤檢測兩種蛋白對判斷胃癌患者的預後具有一定的臨床意義.
목적 탐토β-련배단백(β-catenin)여증식세포핵항원(proliferating cell nuclear antigen,PCNA)재위암조직중적표체,연구기여환자림상병리인소급예후적관계.방법 선취2000년1월지2006년12월간126례수술획득적위암조직화30례정상위조직,구건조직심편,응용면역조화기술검측β-catenin화PCNA재위암급위정상조직적표체,분석기여위암환자림상병리인소급예후적관계.결과 β-catenin화PCNA재위암조직중적양성표체솔분별위96.7％화99.2％,고우정상위조직,차이유통계학의의(x2=41.082,P=0;x2=97.692,P=0);응용ROC곡선검측발현PCNA대위암화정상위조직적감별우우β-catenin,전자적령민도、특이도급부합솔분별위84.4％、87.5％급84.6％.β-catenin여림파결전이유관(x2=6.107.P=0.04),PCNA여병리분형유관(x2=9.71.P=0.02).β-catenin화PCNA재위암조직중적표체정정상관(r=0.24,P=0.005).다인소분석현시위암환자적TNM분기급β-catenin화PCNA균양성표체시영향환자생존적독립인소(P=0、P=0.04),이β-catenin、PCNA적단독양성표체병불시영향위암환자생존적독립인소.결론 β-catenin화PCNA재위암조직중고표체.PCNA대위암화정상위조직적감별우우β-catenin,연합검측량충단백대판단위암환자적예후구유일정적림상의의.
Objective To investigate the expression of β-catenin and PCNA,and its relationship with clinical parameters and prognosis.Methods Tissue microarray technology and immunohistochemical method were used to detect the expression of β-catenin and PCNA in gastric carcinoma and normal tissue collected from January 2000 to December 2006.Data were analyzed by SPSS 19.0.Results The percentage of expression of β-catenin and PCNA in gastric carcinoma is 96.7％,99.2％,respectively,and higher than the normal gastric tissue (x2 =41.082,P =0;x2 =97.692,P =0).ROC curve was used to find that PCNA was better than β-catenin to discriminate gastric carcinoma from normal gastric tissue in sensitivity,specificity,coincidence rate(84.4％,87.5％,84.6％).The expression of β-catenin is related to lymph node metastasis in gastric cancer (x2 =6.107,P =0.04).The expression of PCNA is related to pathological type (x2 =9.71,P =0.02).The expression of β-catenin and PCNA in gastric carcinoma were positively correlated (r =0.24,P =0.005).Multivariate analysis showed that TNM staging and both positive β-catenin and PCNA were independent factors impacting on the overall survival rate of patient with gastric cancer.Conclusions The expression of β-catenin and PCNA in gastric carcinoma is signifrcandy high.PCNA is more important than β-catenin in discriminating gastric carcinoma from normal gastric tissue.Combined detection of the two kinds of protein have some clinical significance in predicting the prognosis of patients with early gastric cancer.
