CAJ | 학술논문

目的：探讨木犀草素对高脂诱导的 ApoE-／-小鼠非酒精性脂肪肝及动脉粥样硬化的改善作用。方法：采用高脂饲料喂养诱导非酒精性脂肪肝和动脉粥样硬化模型，木犀草素低、高剂量（70，140 mg／kg）灌胃给药，每天1次，连续12周。给药结束后，检测血清血脂四项、超氧化物歧化酶（SOD）、丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH-Px），肝脏匀浆检测谷丙转氨酶、天冬氨酸氨基转移酶（AST）；观察肝脏组织 HE 染色和油红 O 染色以及主动脉根部的油红 O 染色；通过 TUNEL试验检测肝脏细胞的凋亡；应用免疫组化检测肝脏 bcl-2和 caspase-3的表达。结果：木犀草素能够使肝脏 ALT、AST 水平降低；提高血清中 SOD、GSH-Px，降低血清 MDA 的表达；改善肝脏中脂质堆积以及脂肪泡的形成，减少主动脉根部的脂质堆积；降低 TUNEL 阳性细胞数，以及提高肝脏中 bcl-2和减少 caspase-3的表达。结论：本研究表明木犀草素可通过减少氧化损伤来改善高脂诱导的 ApoE-／-小鼠非酒精性脂肪肝及动脉粥样硬化。
목적：탐토목서초소대고지유도적 ApoE-／-소서비주정성지방간급동맥죽양경화적개선작용。방법：채용고지사료위양유도비주정성지방간화동맥죽양경화모형，목서초소저、고제량（70，140 mg／kg）관위급약，매천1차，련속12주。급약결속후，검측혈청혈지사항、초양화물기화매（SOD）、병이철（MDA）、곡광감태과양화물매（GSH-Px），간장균장검측곡병전안매、천동안산안기전이매（AST）；관찰간장조직 HE 염색화유홍 O 염색이급주동맥근부적유홍 O 염색；통과 TUNEL시험검측간장세포적조망；응용면역조화검측간장 bcl-2화 caspase-3적표체。결과：목서초소능구사간장 ALT、AST 수평강저；제고혈청중 SOD、GSH-Px，강저혈청 MDA 적표체；개선간장중지질퇴적이급지방포적형성，감소주동맥근부적지질퇴적；강저 TUNEL 양성세포수，이급제고간장중 bcl-2화감소 caspase-3적표체。결론：본연구표명목서초소가통과감소양화손상래개선고지유도적 ApoE-／-소서비주정성지방간급동맥죽양경화。
Objective:This study was to explore the possible effects of luteolin on ApoE-/-mice with non-alcoholic steatohepatitis induced by high fat diet as well as the efficacy on atherosclerosis.Methods:The model was induced by high fat diet,and luteolin were administered by gastric perfusion at 70 and 1 40 mg/kg qd for 1 2 weeks.The serum level of superoxide dismutase(SOD), malondialdehyde(MDA),glutathion peroxidase(GSH-Px)were measured.The contents of alanine transaminase(ALT),aspartate aminotransferase(AST)were measured in liver tissue of grout.The pathological changes of liver tissue were examined by HE stai-ning and the lipid accumulation in the liver and aortic root was tested by oil red O staining.The apoptotic hepatocytes were detec-ted by TUNEL assay and the expression levels of Bcl-2 and caspase-3 were observed by immunohistochemisty.Results:The results showed that Luteolin(70,1 40 mg/kg)improved the liver function significantly through reducing the level of ALT,AST,and in-creasing the content of SOD,GSH-Px and Bcl-2 while reducing the content of MDA,lipid accumulation,hepatic steatosis, TUNEL-positive cells and the expression of caspase-3.Conclusion:The results suggest that Luteolin may inhibit the liver injury and atherosclerosis induced by high fat diet by decreasing the oxidative stress.