CAJ | 학술논문

为了进一步分析玉米基因功能及基因转化，并为玉米抗虫提供种质资源，利用超声波辅助花粉介导法，将抗虫基因 Cry1Ac 导入玉米优良自交系昌7‐2中，获得大量的转基因植株。结果表明：转基因植株经过草铵膦抗性筛选、PCR 检测、BT 蛋白试纸条检测和室内抗虫性鉴定等分析，最后获得高抗玉米螟的转 BtCry1Ac基因株系9个。培育出了高抗玉米螟的种质资源材料，同时也建立了超声波辅助花粉介导转化玉米的高效转化体系，该方法是简捷、快速有效、无基因型限制的植物转化方法。
위료진일보분석옥미기인공능급기인전화，병위옥미항충제공충질자원，이용초성파보조화분개도법，장항충기인 Cry1Ac 도입옥미우량자교계창7‐2중，획득대량적전기인식주。결과표명：전기인식주경과초안련항성사선、PCR 검측、BT 단백시지조검측화실내항충성감정등분석，최후획득고항옥미명적전 BtCry1Ac기인주계9개。배육출료고항옥미명적충질자원재료，동시야건립료초성파보조화분개도전화옥미적고효전화체계，해방법시간첩、쾌속유효、무기인형한제적식물전화방법。
In order to further analyze the gene functions and transformation of maize ,and provide germplasm re‐sources for maize insect‐resistant .The insect resistant Cry1Ac gene was used to transfer into maize inbred lines Chang 7‐2 ,through the method of ultrasonic‐assisted pollen mediated transformation ,more transgenic‐maize plantlets were obtained .The results showed that the transgenic plants were detected by PCR detection , Bt protein strip and raising insect assay in laboratory .Nine elite transgenic lines with high resistance to Ostrinia nubilalis were developed .In conclusion ,it not only provided germplasm resources for maize beeding but also es‐tablished the ultrasonic processing pollen mediated maize genetic transformation method ,which was a simple , rapid and effective method for the transformation of plants .