CAJ | 학술논문

为了获得水稻抗寒育种的基因资源，将腊梅花硫酯酶基因（Cp FA TB）转入水稻中，并对其后代进行了耐冷性鉴定。结果表明：转基因水稻低温下发芽率与常温发芽率比值为98％，与对照相比有所提高，达0．01水平显著差异；幼芽5℃存活率最高为98．41％，与对照达0．001水平显著差异；芽期在经历低温后比对照能较快地恢复生长；孕穗期经低温处理后，穗长、穗重、千粒重等指标无显著差异，有效分蘖率最高为76．928％，空粒率最低为10．02％，与对照达0．001水平显著差异。说明外源基因 Cp FA TB 在一定程度上提高了水稻的耐冷性。
위료획득수도항한육충적기인자원，장석매화류지매기인（Cp FA TB）전입수도중，병대기후대진행료내랭성감정。결과표명：전기인수도저온하발아솔여상온발아솔비치위98％，여대조상비유소제고，체0．01수평현저차이；유아5℃존활솔최고위98．41％，여대조체0．001수평현저차이；아기재경력저온후비대조능교쾌지회복생장；잉수기경저온처리후，수장、수중、천립중등지표무현저차이，유효분얼솔최고위76．928％，공립솔최저위10．02％，여대조체0．001수평현저차이。설명외원기인 Cp FA TB 재일정정도상제고료수도적내랭성。
In order to acquire cold‐tolerance resourses of rice for breeding ,Cp FA TB (Chimonanthus praecox chloroplast acyl‐ACP thioesterase)was transfered into japonica rice ,and the ability of cold‐tolerance of the third GMOs’ generation was tested .The results showed that the ratio of the germination rate under low‐temperature treatment and regular temperature was 98% that significantly higher than the non‐GMO cultivar .The survival rate of seedling at 5 ℃ was 98 .41% ,reached highly significant level .The GMOs recovered quicker than non‐GMO cultivar after low‐temperature treatment in sprouting stage .There was no significant difference with sta‐tus like TKW ,average panicle weight and length .But the effective tillering rate and empty seeds rate were in highly significant level difference after low‐temperature treatment in booting stage .All results certificated that the Cp FA TB improved the ability of cold‐tolerance of rice to a certain degree .