临床消化病杂志
臨床消化病雜誌
림상소화병잡지
Chinese Journal of Clinical Gastroenterology
2015年
4期
193-196
,共4页
徐言%聂玉强%廖德贵%陈婷
徐言%聶玉彊%廖德貴%陳婷
서언%섭옥강%료덕귀%진정
肝细胞癌%他扎罗汀诱导基因3%分子靶向治疗
肝細胞癌%他扎囉汀誘導基因3%分子靶嚮治療
간세포암%타찰라정유도기인3%분자파향치료
hepatocellular carcinoma%tazarotene-induced gene 3%molecular therapy target
[目的]探讨他扎罗汀诱导基因3（TIG3）在肝癌中的表达情况及其抑制作用。[方法]在临床病理组织标本中，通过免疫组化检测肝癌组织和正常肝组织 TIG3、核增殖抗原（Ki67）蛋白表达情况。在细胞系中，采用实时定量PCR技术，检测TIG3在正常肝细胞与肝癌细胞中表达情况；利用脂质体2000向 TIG3内在表达量最低的细胞中，转染TIG3过表达质粒，通过荧光定量PCR检测转染效果，同时再通过MTT比色法探讨过表达 TIG3对肝癌细胞活力的影响。[结果]在临床病理组织标本中，T IG3在肝癌组织中低表达，且与Ki67的表达呈负相关；细胞功能研究发现，过表达T IG3可使肝癌细胞活力下降，肝癌细胞生长增殖受到抑制。[结论]T IG3在肝癌组织中低表达，且可抑制肝癌生长增殖，因此T IG3可能作为肝癌分子靶向治疗新靶点。
[目的]探討他扎囉汀誘導基因3（TIG3）在肝癌中的錶達情況及其抑製作用。[方法]在臨床病理組織標本中，通過免疫組化檢測肝癌組織和正常肝組織 TIG3、覈增殖抗原（Ki67）蛋白錶達情況。在細胞繫中，採用實時定量PCR技術，檢測TIG3在正常肝細胞與肝癌細胞中錶達情況；利用脂質體2000嚮 TIG3內在錶達量最低的細胞中，轉染TIG3過錶達質粒，通過熒光定量PCR檢測轉染效果，同時再通過MTT比色法探討過錶達 TIG3對肝癌細胞活力的影響。[結果]在臨床病理組織標本中，T IG3在肝癌組織中低錶達，且與Ki67的錶達呈負相關；細胞功能研究髮現，過錶達T IG3可使肝癌細胞活力下降，肝癌細胞生長增殖受到抑製。[結論]T IG3在肝癌組織中低錶達，且可抑製肝癌生長增殖，因此T IG3可能作為肝癌分子靶嚮治療新靶點。
[목적]탐토타찰라정유도기인3（TIG3）재간암중적표체정황급기억제작용。[방법]재림상병리조직표본중，통과면역조화검측간암조직화정상간조직 TIG3、핵증식항원（Ki67）단백표체정황。재세포계중，채용실시정량PCR기술，검측TIG3재정상간세포여간암세포중표체정황；이용지질체2000향 TIG3내재표체량최저적세포중，전염TIG3과표체질립，통과형광정량PCR검측전염효과，동시재통과MTT비색법탐토과표체 TIG3대간암세포활력적영향。[결과]재림상병리조직표본중，T IG3재간암조직중저표체，차여Ki67적표체정부상관；세포공능연구발현，과표체T IG3가사간암세포활력하강，간암세포생장증식수도억제。[결론]T IG3재간암조직중저표체，차가억제간암생장증식，인차T IG3가능작위간암분자파향치료신파점。
Objective]To explore the expression of tazarotene‐induced gene 3(TIG3)in hepatocellular carcinoma and its inhibitory effect. [Methods]With the immunohistochemistry of clinical specimens ,TIG3 and Ki67 protein was detected in hepatocellular carcinoma tissues and adjacent normal tissues.Meanwhile , in cell lines level ,the expression of TIG3 in normal liver cells and liver cancer cells was determined by real time quantitative PCR.The cell which expressed the minimum quantity of TIG3 was transfected transiently with TIG3 overexpressed plasmid by using lipofectamine 2000. After cell transfection ,real time quantitative PCR was used to evaluate the effect of transfection. At the same time ,the role of TIG3 overexpression in the liver cancer cell viability was evaluated by MTT assay.[Results]In the clinical samples ,TIG3 was low expression in hepatocellular carcinoma tissues ,and it was negatively related to the expression of Ki‐67 pro‐tein in hepatocellular carcinoma tissue. In the subsequent experiment of cell function ,overexpression of TIG3 could make the liver cancer cell viability decrease and inhibit the growth of liver cancer cell.[Conclu‐sion]TIG3 is low expression in hepatocellular carcinoma ,and it can inhibit the proliferation of HCC ,so TIG3 can be used as a new molecular therapy target of HCC.
