CAJ | 학술논문

目的：观察兔胫骨骨缺损修复过程中应用基因重组人生长激素的促成骨效果，探讨其在骨缺损愈合中的作用。方法：新西兰大白兔15只（4～6月大，2．5～3．5 kg，雌雄不限），于双侧胫骨制备直径3 mm，深6 mm的骨缺损模型；右侧为实验组，在胫骨缺损区注入包含1 IU基因重组人生长激素自体血凝块0．1 mL；左侧为对照组，在胫骨缺损区注入等量自体血凝块。分别于术后2、4和8周进行骨缺损大体观察、X线影像学、组织学检测以及新生骨占缺损面积百分比的测定。结果：术后各时间点的大体观察、X线影像学、组织学切片观察显示，实验组和对照组均有不同程度的新骨形成；在术后2周和4周各组新生骨密度低于正常骨组织，实验组新生骨面积百分比均大于对照组（P＜0．05）。术后8周时，实验组缺损表面可见连续的皮质骨形成，植骨区新骨形成并趋于成熟，对照组修复效果次之；两组新生骨面积百分比无显著性差异（P＞0．05）。结论：在兔胫骨缺损修复中，基因重组人生长激素能够加速骨缺损区骨小梁的形成和成熟，并对新骨形成有一定促进作用。
목적：관찰토경골골결손수복과정중응용기인중조인생장격소적촉성골효과，탐토기재골결손유합중적작용。방법：신서란대백토15지（4～6월대，2．5～3．5 kg，자웅불한），우쌍측경골제비직경3 mm，심6 mm적골결손모형；우측위실험조，재경골결손구주입포함1 IU기인중조인생장격소자체혈응괴0．1 mL；좌측위대조조，재경골결손구주입등량자체혈응괴。분별우술후2、4화8주진행골결손대체관찰、X선영상학、조직학검측이급신생골점결손면적백분비적측정。결과：술후각시간점적대체관찰、X선영상학、조직학절편관찰현시，실험조화대조조균유불동정도적신골형성；재술후2주화4주각조신생골밀도저우정상골조직，실험조신생골면적백분비균대우대조조（P＜0．05）。술후8주시，실험조결손표면가견련속적피질골형성，식골구신골형성병추우성숙，대조조수복효과차지；량조신생골면적백분비무현저성차이（P＞0．05）。결론：재토경골결손수복중，기인중조인생장격소능구가속골결손구골소량적형성화성숙，병대신골형성유일정촉진작용。
Objective:The purpose of the study was to observe the effect of topical use of recombinant human growth hormone (rhGH) in the repair process of tibial bone defects in rabbits and explore the role of rhGH in bone healing. Method:The bilateral tibial defects models (3 mm in diameter,and l 0 mm in depth)were established in 15 New Zealand rabbits (4~6 months old,2.5~3.5 kg,no gender limitations). The right side which treated with1 IU rhGH involved in 0.1 mL autologus blood clot was experimental group,and the left side which treated with 0.1 mL autologus blood clot was control group. In postoperative 2nd,4th,8th week,general observation,X-ray radiograph,histological examination,the osteogenesis effect in the defect region were analyzed. Result:In each postoperative time point,the experimental and control groups showed new bone formation. At postoperative 2nd and 4th week,the densities of the new bones were lower than normal bones,the quantities of new bones were larger in the experimental groups than in the control groups(P<0.05).At postopera-tive 8th week,continuous cortical bone formations were seen on the surfaces in the experimental group,new bone formed and tended to be mature,but those in the control group were not as good. There was no significant difference between the two groups in term of quantity of new bone area. Conclusion:These findings indicate that the topical use of rhGH could acceler-ate the formation and maturation of trabecular bone and have some effect on new bone information.