CAJ | 학술논문

目的：观察姜黄素干预前后肝星状细胞（HSC）中髓样分化因子88（MyD88）蛋白的变化水平，以探讨姜黄素抗肝纤维化的相关机制。方法构建pCMV-Myc-MyD88重组质粒，将人肾上皮细胞293T细胞及HSC-T6细胞分别设为空白对照组、MyD88过表达组、姜黄素+MyD88过表达组，其中MyD88过表达组给予转染pCMV-Myc-MyD88重组质粒72 h，姜黄素+MyD88过表达组在转染48 h后加入姜黄素继续作用24 h。采用Western blot法检测MyD88蛋白表达。结果①转染pCMV-myc-MyD88重组质粒48 h后，MyD88在293T细胞及HSC中表达均明显增高（P<0.05），且经姜黄素处理后两类细胞中表达量明显降低（P<0.05）。②姜黄素处理后MyD88表达量较MyD88质粒转染组明显降低（P<0.05）。结论姜黄素可能通过抑制肝星状细胞MyD88蛋白表达，阻断MyD88依赖性信号通路的转导而发挥抗肝纤维化的作用。
목적：관찰강황소간예전후간성상세포（HSC）중수양분화인자88（MyD88）단백적변화수평，이탐토강황소항간섬유화적상관궤제。방법구건pCMV-Myc-MyD88중조질립，장인신상피세포293T세포급HSC-T6세포분별설위공백대조조、MyD88과표체조、강황소+MyD88과표체조，기중MyD88과표체조급여전염pCMV-Myc-MyD88중조질립72 h，강황소+MyD88과표체조재전염48 h후가입강황소계속작용24 h。채용Western blot법검측MyD88단백표체。결과①전염pCMV-myc-MyD88중조질립48 h후，MyD88재293T세포급HSC중표체균명현증고（P<0.05），차경강황소처리후량류세포중표체량명현강저（P<0.05）。②강황소처리후MyD88표체량교MyD88질립전염조명현강저（P<0.05）。결론강황소가능통과억제간성상세포MyD88단백표체，조단MyD88의뢰성신호통로적전도이발휘항간섬유화적작용。
Objective To observe the expression difference of myeloid differentiation factor 88(MyD88) protein in hepatic stellate cell (HSC) treated with curcumin, and to investigate the related mechanism of the an-ti-fibrotic effect of curcumin. Methods 293T cell and HSC cell were individually divided into control group, vector of MyD88 plasmid group,and curcumin+vectors of MyD88 plasmid group. The expression of MyD88 was detected by Western blot. Results ①After transfection of pCMV-Myc-MyD88, the expression of MyD88 was significantly increased (P<0.05), while the expression of MyD88 in curcumin+vectors of MyD88 plas-mid group was significantly decreased than vectors of MyD88 plasmid group (P<0.05). ②After transfection of pCMV-Myc-MyD88 HSC, the expression of MyD88 was significantly increased (P<0.05). The expression of MyD88 in curcumin+vectors of MyD88 plasmid group was significantly decreased compared with vectors of MyD88 plasmid group(P<0.05). Conclusions Curcumin might prevent liver fibrosis by inhabiting the ex-pression of MyD88 protein and blocking MyD88-dependent signaling pathway.