CAJ | 학술논문

目的：增殖诱导配体（APRIL）与结直肠癌细胞对5-氟尿嘧啶（5-FU）的耐药性密切相关。我们前期已利用噬菌体随机肽库技术成功筛选并人工合成与sAPRIL有高亲和力的结合肽。本研究旨在探讨sAPRIL结合肽对结直肠癌LOVO细胞5-FU化疗敏感性的影响。方法将LOVO细胞分为PBS组、5-FU组、sAPRIL结合肽+5-FU组。采用CCK-8法检测细胞增殖、流式细胞仪测定细胞周期及细胞凋亡。结果5-FU组和sAPRIL结合肽+5-FU组增殖抑制率分别为（37.62±7.07）%、（63.05±2.15）%；流式细胞仪检测各组的细胞周期显示5-FU组阻滞在S期，sAPRIL结合肽+5-FU组阻滞在G0/G1期和S期，且S期阻滞效应显著强于5-FU组；三组细胞凋亡率分别为（0.21±0.09）%、（12.80±0.30）%、（17.1±0.66）%。结论 sAPRIL结合肽能增强结直肠癌LOVO细胞对5-FU的化疗敏感性，有望成为结直肠癌靶向治疗的新型候选制剂。
목적：증식유도배체（APRIL）여결직장암세포대5-불뇨밀정（5-FU）적내약성밀절상관。아문전기이이용서균체수궤태고기술성공사선병인공합성여sAPRIL유고친화력적결합태。본연구지재탐토sAPRIL결합태대결직장암LOVO세포5-FU화료민감성적영향。방법장LOVO세포분위PBS조、5-FU조、sAPRIL결합태+5-FU조。채용CCK-8법검측세포증식、류식세포의측정세포주기급세포조망。결과5-FU조화sAPRIL결합태+5-FU조증식억제솔분별위（37.62±7.07）%、（63.05±2.15）%；류식세포의검측각조적세포주기현시5-FU조조체재S기，sAPRIL결합태+5-FU조조체재G0/G1기화S기，차S기조체효응현저강우5-FU조；삼조세포조망솔분별위（0.21±0.09）%、（12.80±0.30）%、（17.1±0.66）%。결론 sAPRIL결합태능증강결직장암LOVO세포대5-FU적화료민감성，유망성위결직장암파향치료적신형후선제제。
Objective This study aimed to verify if the sAPRIL-binding peptide can enhance chemo-sensitivity of 5-FU on colorectal cancer cells. Methods LOVO cells were divided into three groups: PBS, 5-FU, and sAPRIL-binding peptide plus 5-FU. The cell growth inhibition rate was detected by CCK-8 assay, and the cell cycle and cell apoptosis were detected by flow cytometry. Results The cell growth inhibition rate was (0±0)%in PBS group, (37.62±7.07)%in 5-FUgroup, (63.05±2.15)%in sAPRIL-binding peptide plus 5-FU group, respectively. In 5-FU group, cell cycle was arrested at S phase;and in sAPRIL-binding peptide plus 5-FU group, in sAPRIL-binding peptide plus 5-FU group, cell cycle was arrested at both G0/G1 and S phases ( more markedly at S phase compared with 5-FU alone). The percentage of early apoptotic cells was (0.21±0.09)%in PBS group, (12.80±0.30)%in 5-FUgroup, and (17.1±0.66)%in sAPRIL-binding peptide plus 5-FU group, respectively. Conclusions The sAPRIL-binding peptide can enhance chemo-sensitivity of 5-FU on colorectal cancer LOVO cells. This peptide might be a new approach for molecular targeted therapy of colorectal cancer.