CAJ | 학술논문

对于角膜缘干细胞(LSCs)缺乏导致的眼表疾病,角膜移植是较好的治疗方法,传统角膜移植术的开展受供体来源匮乏、术后排斥反应等因素的影响.构建组织工程角膜上皮进行移植很大程度上解决了供体来源和术后免疫排斥的问题,成为重要和有效的治疗方法,而种子细胞的选择是手术成功的关键.随着组织工程技术和细胞培养技术的不断发展,组织工程角膜种子细胞方面的研究越来越多,种子细胞的可选择性也越来越大,如胚胎干细胞(ESCs)、LSCs等研究已经逐步展开.就目前体外重建组织工程角膜上皮研究中使用的种子细胞及其近年来的相关研究进展进行综述.
대우각막연간세포(LSCs)결핍도치적안표질병,각막이식시교호적치료방법,전통각막이식술적개전수공체래원궤핍、술후배척반응등인소적영향.구건조직공정각막상피진행이식흔대정도상해결료공체래원화술후면역배척적문제,성위중요화유효적치료방법,이충자세포적선택시수술성공적관건.수착조직공정기술화세포배양기술적불단발전,조직공정각막충자세포방면적연구월래월다,충자세포적가선택성야월래월대,여배태간세포(ESCs)、LSCs등연구이경축보전개.취목전체외중건조직공정각막상피연구중사용적충자세포급기근년래적상관연구진전진행종술.
Keratoplasty is a choice for the treatment of ocular surface diseases caused by corneal limbal stem cells (LSCs) deficiency.The application of traditional keratoplasty is limited by avaibility of donor corneas and allograft rejection.Constructruction of tissue engineering corneal epithelium provides an important and effective approach to the transplantation of cornea,because it can solve the lack of donor corneas and avoid allograft rejection following keratoplasty.However,the selection of the seed cells is crucial to corneal tissue engineering.What is more,the research of seed cells is becoming more and more widespread,just like embryonic stem cells (ESCs) and LSCs.This article summarized the selection of seed cells and the progress of tissue-engineered human corneal epithelium.