CAJ | 학술논문

目的：利用静电纺丝法制备载盐酸多西环素（DOX）纳米纤维膜片，评价其作为局部药物治疗与GTR／GBR技术的结合，在治疗种植体周围炎方面的可行性的相关基础研究。方法：用静电纺丝法制备载DOX的聚己内酯（PCL）纳米纤维膜。扫描电子显微镜观察纤维膜的表面形貌、分析药物对纤维形貌的影响，差示量热扫描法（DSC）分析药物在纤维中的分布状态，琼脂扩散实验检测载药膜片对牙龈卟啉单胞菌（Pg）和伴放线放线杆菌（Aa）的抑菌作用。结果：制备的DOX－PCL纳米纤维表面光滑，直径分布均匀，随着DOX的载药量由15％增加到25％，纤维直径由247．17 nm降低到181．34 nm；同时以无定形分子形式分布于纳米纤维中的DOX对Aa和Pg均具有明显的抑菌作用。结论：作为药物治疗与GTR／GBR技术的结合，静电纺丝法制备的载药GTR／GBR膜在种植体周围炎的治疗方面具有良好的应用前景。
목적：이용정전방사법제비재염산다서배소（DOX）납미섬유막편，평개기작위국부약물치료여GTR／GBR기술적결합，재치료충식체주위염방면적가행성적상관기출연구。방법：용정전방사법제비재DOX적취기내지（PCL）납미섬유막。소묘전자현미경관찰섬유막적표면형모、분석약물대섬유형모적영향，차시량열소묘법（DSC）분석약물재섬유중적분포상태，경지확산실험검측재약막편대아간계람단포균（Pg）화반방선방선간균（Aa）적억균작용。결과：제비적DOX－PCL납미섬유표면광활，직경분포균균，수착DOX적재약량유15％증가도25％，섬유직경유247．17 nm강저도181．34 nm；동시이무정형분자형식분포우납미섬유중적DOX대Aa화Pg균구유명현적억균작용。결론：작위약물치료여GTR／GBR기술적결합，정전방사법제비적재약GTR／GBR막재충식체주위염적치료방면구유량호적응용전경。
Objective:To prepare a doxycycline hydrochloride(DOX)-loaded nanofibrous membrane by electrospining method. Evaluate its relative foundational properties for peri-implantitis therapy as the combination of local drug treatment and GTR/GBR surgery. Method:Different groups' DOX-loaded PCL nanofibrous membranes were prepared by eletrospun. Then we observed the morphology and the diameter distribution of nanofibers,investigated the manner of DOX in drug-load-ed nanofibers and its bioactivity against Aggregatibacter actinomycetemcomitans (Aa) and Porphyromonas gingivalis(Pg). Result:DOX-loaded PCL nanofibers were uniform distributed and the surface of fibers were smooth. Fibrous average diame-ters decreased from 247.16 nm to 181.34nm with the drug proportion increasing from 15%to 25%w/w. Differential scan-ning calorimetry (DSC) test showed DOX appeared an amorphous manner in the polymer matrix. And DOX-loaded mem-branes had an obvious inhibited effect on Aggregatibacter actinomycetemcomitans (Aa) and Porphyromonas gingivalis(Pg), respectively. Conclusion:As the combination of local drug treatment and GTR/GBR surgery,electrospinning DOX-loaded PCL nanofibrous membrane has a well prospect on the field of peri-implantitis therapy.