世界中医药
世界中醫藥
세계중의약
World Chinese Medicine
2015年
8期
1246-1249
,共4页
古丽娜尔·叶尔森那力%木拉提·克扎衣别克%江阿古丽·艾山%江安努尔·这依肯%地达尔·巴合提坚
古麗娜爾·葉爾森那力%木拉提·剋扎衣彆剋%江阿古麗·艾山%江安努爾·這依肯%地達爾·巴閤提堅
고려나이·협이삼나력%목랍제·극찰의별극%강아고려·애산%강안노이·저의긍%지체이·파합제견
哈医祛风膏%去氢骆驼蓬碱%骆驼莲碱骆驼蓬碱%高效液相色谱法%疗效
哈醫祛風膏%去氫駱駝蓬堿%駱駝蓮堿駱駝蓬堿%高效液相色譜法%療效
합의거풍고%거경락타봉감%락타련감락타봉감%고효액상색보법%료효
Hayi wind-dispelling ointment%Harmine%Harmaline%HPLC method%Clinical efficacy
目的：建立高效液相色谱法（HPLC）测定哈医祛风膏中去氢骆驼蓬碱和骆驼蓬碱的含量及临床疗效观察。方法：采用高效液相法测定含量，色谱柱为 Agilent TC-C18（4．6 mm ×250 mm，5μm），以甲醇：0．01 moL／L 硫酸铵溶液（37：63，用三乙胺调 pH 至4．0）为流动相，流速为1 mL／min，柱温：25℃，检测波长在320 nm。用完全随机法分实验组和对照组进行比较哈医祛风膏疗效。结果：去氢骆驼蓬碱的线性范围为12～83μg（r ＝0．9998，n ＝6）平均回收率为100．14％，RSD 值为1．33％、骆驼蓬碱的线性范围为12～83μg（r ＝0．9998，n ＝6）平均回收率为98．89％，RSD 值为1．77％。疗效比较治疗组的总有效率为97．3％、对照组的总有效率为90．1％，2组有显著性差异，P ＜0．01。表明治疗组总体疗效明显优于对照组。结论：高效液相色谱法方法简便，准确，可靠，可以用于哈医祛风膏中去氢骆驼蓬碱和骆驼蓬碱的含量测定。并哈医祛风膏治疗颈肩腰腿痛的效果较好、疗效确切、安全性良好，可降低患者复发率，可考虑在临床上推广。
目的：建立高效液相色譜法（HPLC）測定哈醫祛風膏中去氫駱駝蓬堿和駱駝蓬堿的含量及臨床療效觀察。方法：採用高效液相法測定含量，色譜柱為 Agilent TC-C18（4．6 mm ×250 mm，5μm），以甲醇：0．01 moL／L 硫痠銨溶液（37：63，用三乙胺調 pH 至4．0）為流動相，流速為1 mL／min，柱溫：25℃，檢測波長在320 nm。用完全隨機法分實驗組和對照組進行比較哈醫祛風膏療效。結果：去氫駱駝蓬堿的線性範圍為12～83μg（r ＝0．9998，n ＝6）平均迴收率為100．14％，RSD 值為1．33％、駱駝蓬堿的線性範圍為12～83μg（r ＝0．9998，n ＝6）平均迴收率為98．89％，RSD 值為1．77％。療效比較治療組的總有效率為97．3％、對照組的總有效率為90．1％，2組有顯著性差異，P ＜0．01。錶明治療組總體療效明顯優于對照組。結論：高效液相色譜法方法簡便，準確，可靠，可以用于哈醫祛風膏中去氫駱駝蓬堿和駱駝蓬堿的含量測定。併哈醫祛風膏治療頸肩腰腿痛的效果較好、療效確切、安全性良好，可降低患者複髮率，可攷慮在臨床上推廣。
목적：건립고효액상색보법（HPLC）측정합의거풍고중거경락타봉감화락타봉감적함량급림상료효관찰。방법：채용고효액상법측정함량，색보주위 Agilent TC-C18（4．6 mm ×250 mm，5μm），이갑순：0．01 moL／L 류산안용액（37：63，용삼을알조 pH 지4．0）위류동상，류속위1 mL／min，주온：25℃，검측파장재320 nm。용완전수궤법분실험조화대조조진행비교합의거풍고료효。결과：거경락타봉감적선성범위위12～83μg（r ＝0．9998，n ＝6）평균회수솔위100．14％，RSD 치위1．33％、락타봉감적선성범위위12～83μg（r ＝0．9998，n ＝6）평균회수솔위98．89％，RSD 치위1．77％。료효비교치료조적총유효솔위97．3％、대조조적총유효솔위90．1％，2조유현저성차이，P ＜0．01。표명치료조총체료효명현우우대조조。결론：고효액상색보법방법간편，준학，가고，가이용우합의거풍고중거경락타봉감화락타봉감적함량측정。병합의거풍고치료경견요퇴통적효과교호、료효학절、안전성량호，가강저환자복발솔，가고필재림상상추엄。
Objective:To study the content and clinical effect of Harmine and Harmaline in Hayi wind-dispelling ointment by HPLC method.Methods:The sample was separated on Agilent TC-C1 8 (4.6 mm ×250 mm,5 μm).CH30H of 0.01 moL·L -1 and(NH3 )2 SO4 (63∶37 pH regulated to 4.0 by triethylamine)were used as the mobile phase,with flow rate at 1 mL·min -1 ,col-umn temperature at 25 ℃,the determining wavelength at 320nm.Complete random method was used to divide control group and experimental group to compare the efficacy of Hayi wind-dispelling ointment.Results:Harmine calibration curve was linear over the range of 1 2-83 μg(r =0.9998,n =6).The average recovery rate was 1 00.1 4%,RSD was 1 .33%.Harmaline calibration curve was linear over the range of 1 2-83 μg(r =0.9998,n =6).The recovery rate was 98.89%,RSD was 1 .77%.The total ef-fective rate of treatment group was 97.3%,followed by the control group at 90.1 %.There was significant difference between the two groups(P <0.01 ),which indicates that the overall curative effect of treatment group was obviously better than that of the con-trol group.Conclusion:The HPLC method is simple,accurate,and reliable,which can be applied to determine the content of harmine and harmaline in Hayi wind-dispelling ointment.Hayi wind-dispelling ointment is effective and safe for the treatment of shoulder,back and leg pain and can reduce the relapse rate of patients,thus can be further promoted in clinical practice.
