CAJ | 학술논문

目的:研究不同摩擦力刺激对经脉筋膜结缔组织成纤维细胞的整合素β1亚基和细胞骨架中微丝力学信号传导通路的影响.方法:体外培养经脉相关筋膜结缔组织成纤维细胞,不同强度摩擦力单次或多次刺激后检测细胞整合素β1表达情况,观察其微丝形态学变化.结果:⑴经脉筋膜结缔组织成纤维细胞可以通过整合素β1感受摩擦力学刺激,单次摩擦力刺激以中重度刺激整合素β1表达量高,多次摩擦力刺激以轻度刺激整合素β1表达量高,重度刺激整合素β1表达量甚至低于空白组;析因方差分析表明摩擦力的刺激强度因素和刺激次数因素均是整合素β1表达量改变的原因,且两者存在交互作用(P<0.01).⑵经脉相关筋膜结缔组织成纤维细胞感受不同摩擦力刺激能引起细胞骨架中微丝的构型改变,单次及多次刺激微丝形态结构均呈渐变特征,且多次刺激较单次刺激明显.结论:整合素β1亚基和细胞骨架微丝的表达变化规律可能从生物力学角度揭示了经脉相关组织细胞对针推摩擦力刺激的物理学信号感受和传导机制.
목적:연구불동마찰력자격대경맥근막결체조직성섬유세포적정합소β1아기화세포골가중미사역학신호전도통로적영향.방법:체외배양경맥상관근막결체조직성섬유세포,불동강도마찰력단차혹다차자격후검측세포정합소β1표체정황,관찰기미사형태학변화.결과:⑴경맥근막결체조직성섬유세포가이통과정합소β1감수마찰역학자격,단차마찰력자격이중중도자격정합소β1표체량고,다차마찰력자격이경도자격정합소β1표체량고,중도자격정합소β1표체량심지저우공백조;석인방차분석표명마찰력적자격강도인소화자격차수인소균시정합소β1표체량개변적원인,차량자존재교호작용(P<0.01).⑵경맥상관근막결체조직성섬유세포감수불동마찰력자격능인기세포골가중미사적구형개변,단차급다차자격미사형태결구균정점변특정,차다차자격교단차자격명현.결론:정합소β1아기화세포골가미사적표체변화규률가능종생물역학각도게시료경맥상관조직세포대침추마찰력자격적물이학신호감수화전도궤제.
Objective:To study the effect of different friction stimulation on fibroblast integrin β1 subunit and cytoskeleton microfilament mechanical signal transduction pathway of meridian fascia connective tissue. Methods:In vitro cultivating meridian fascia connective tissue fibroblasts, after dif-ferent intensity of friction single or repeated stimulation expression of integrin β1 was detected, the microfilament morphological changes were ob-served. Results:⑴Meridians fascia connective tissue fibroblast could feel the friction mechanical stimulation with integrinβ1, expressions of integ-rin β1 with moderate and severe stimulation of single friction stimulation were much higher, expressions of integrin β1 with mild stimulation of re-peated friction stimulation were higher, with severe stimulation was even lower than blank group;factorial analysis of variance showed that stimulus intensity and times of friction were the reasons of integrin β1 expression change, and both with interaction (P<0.01).⑵Meridians fascia connective tissue fibroblast with different friction stimulation could cause the configuration changes of cytoskeleton microfilament, microfilament morphologi-cal structure with single and repeated stimulation both showed gradient characteristic, and repeated stimulation was more significant than single stim-ulation. Conclusion:Expressive disciplinarian of integrin β1 subunit and cytoskeleton microfilament may from the perspective of biomechanics re-veal the physics signal perception and transmission mechanism of meridians related tissue cells on friction stimulation of acupuncture and massage.