按摩与康复医学
按摩與康複醫學
안마여강복의학
Chinese Manipulation & Rehabilitation Medicine
2015年
18期
134-137
,共4页
江瑜%陈波%李程%陈磊%胡铁汉
江瑜%陳波%李程%陳磊%鬍鐵漢
강유%진파%리정%진뢰%호철한
血管内皮细胞%ET-1%PGI2%IFN-β%TNF-α%针灸%推拿%力学刺激
血管內皮細胞%ET-1%PGI2%IFN-β%TNF-α%針灸%推拿%力學刺激
혈관내피세포%ET-1%PGI2%IFN-β%TNF-α%침구%추나%역학자격
vascular endothelial cells%ET-1%PGI2%IFN-β%TNF-α%acupuncture%massage%mechanical stimulation
目的:探讨针灸推拿体外压刺激对血管内皮细胞ET-1、PGI2、IFN-β和TNF-α的影响.方法:体外培养血管内皮细胞,平分为空白对照组、低压力力学刺激组和高压力刺激组,分别予以0kPa、100kPa和200kPa压力刺激,检测细胞外培养液血管活性物质ET-1、PGI2、IFN-β和TNF-α含量.结果:低、高压力刺激组PGI2合成释放量均显著少于空白对照组(P<0.01),高压力刺激组ET-1合成释放多于空白对照组(P<0.05);高压力刺激组IFN-β合成释放量显著多于空白对照组(P<0.05),低、高压力刺激组TNF-α合成释放量均多于空白对照组(P<0.05).结论:压力刺激调节血管内皮细胞血管舒缩活性物质ET-1、PGI2合成释放,促进具有免疫调节功能的细胞因子IFN-β、TNF-α合成释放,可能是针灸推拿发挥"运气血、扶助正气"作用的细胞生物力学原理之一.
目的:探討針灸推拿體外壓刺激對血管內皮細胞ET-1、PGI2、IFN-β和TNF-α的影響.方法:體外培養血管內皮細胞,平分為空白對照組、低壓力力學刺激組和高壓力刺激組,分彆予以0kPa、100kPa和200kPa壓力刺激,檢測細胞外培養液血管活性物質ET-1、PGI2、IFN-β和TNF-α含量.結果:低、高壓力刺激組PGI2閤成釋放量均顯著少于空白對照組(P<0.01),高壓力刺激組ET-1閤成釋放多于空白對照組(P<0.05);高壓力刺激組IFN-β閤成釋放量顯著多于空白對照組(P<0.05),低、高壓力刺激組TNF-α閤成釋放量均多于空白對照組(P<0.05).結論:壓力刺激調節血管內皮細胞血管舒縮活性物質ET-1、PGI2閤成釋放,促進具有免疫調節功能的細胞因子IFN-β、TNF-α閤成釋放,可能是針灸推拿髮揮"運氣血、扶助正氣"作用的細胞生物力學原理之一.
목적:탐토침구추나체외압자격대혈관내피세포ET-1、PGI2、IFN-β화TNF-α적영향.방법:체외배양혈관내피세포,평분위공백대조조、저압력역학자격조화고압력자격조,분별여이0kPa、100kPa화200kPa압력자격,검측세포외배양액혈관활성물질ET-1、PGI2、IFN-β화TNF-α함량.결과:저、고압력자격조PGI2합성석방량균현저소우공백대조조(P<0.01),고압력자격조ET-1합성석방다우공백대조조(P<0.05);고압력자격조IFN-β합성석방량현저다우공백대조조(P<0.05),저、고압력자격조TNF-α합성석방량균다우공백대조조(P<0.05).결론:압력자격조절혈관내피세포혈관서축활성물질ET-1、PGI2합성석방,촉진구유면역조절공능적세포인자IFN-β、TNF-α합성석방,가능시침구추나발휘"운기혈、부조정기"작용적세포생물역학원리지일.
Objective:To investigate the effects of acupuncture and massage on ET-1, PGI2, IFN-βand TNF-αof vascular endothelial cells. Methods:The vascular endothelial cells were cultured external and divided into blank control group, low pressure mechanical stimulation group and high pres-sure stimulation group, respectively with 0kPa, 100kPA, 200kPa pressure stimulation, content of vasoactive substance ET-1, PGI2, IFN-βand TNF-αof extracellular nutrient solution was detected. Results:The synthetize and release of PGI2 in low and high pressure stimulation group were both sig-nificantly less than that in blank control group (P<0.01), and ET-1of high pressure stimulation group was more than that of blank control group (P<0.05);IFN-βof high pressure stimulation group was significantly more than that of blank control group (P<0.05), TNF-αof low and high pressure stimulation group was more than that of blank control group (P<0.05). Conclusion:Pressure stimulation regulates the synthetize and release of vaso-motor active substances ET-1 and PGI2 of vascular endothelial cells, and promotes the synthetize and release of cytokines IFN-βand TNF-αwith im-mune regulating function, which could be one of biomechanical principles of acupuncture and massage playing a role of " carrying qi and blood, strengthening genuine qi".
