CAJ | 학술논문

用自制的微囊藻毒素（Microcystin-LR，MC-LR）单克隆抗体与钥孔血蓝蛋白（KLH）偶联物为胶体金标记底物研究 MC-LR 胶体金免疫检测技术。制备各种粒径的胶体金，最终确定粒径30 nm 的胶体金作为标记抗 MC-LR 单克隆抗体效果最佳；制备半抗原 MC-LR 与 KLH 的偶联物；在硝酸纤维素膜上包被 MC-LR 与KLH 的偶联物作为检测带、包被羊抗鼠 IgG 作为质控带，建立快速检测 MC-LR 的免疫层析试纸条方法，该方法在3～5 min 即可目测判断结果，灵敏度为2 ng·mL－1；依据工艺流程，发明制备了微囊藻毒素 MC-LR 的免疫层析快速检测卡，该卡检测样本中的微囊藻毒素 MC-LR 含量阈值为2 ng·mL－1。
용자제적미낭조독소（Microcystin-LR，MC-LR）단극륭항체여약공혈람단백（KLH）우련물위효체금표기저물연구 MC-LR 효체금면역검측기술。제비각충립경적효체금，최종학정립경30 nm 적효체금작위표기항 MC-LR 단극륭항체효과최가；제비반항원 MC-LR 여 KLH 적우련물；재초산섬유소막상포피 MC-LR 여KLH 적우련물작위검측대、포피양항서 IgG 작위질공대，건립쾌속검측 MC-LR 적면역층석시지조방법，해방법재3～5 min 즉가목측판단결과，령민도위2 ng·mL－1；의거공예류정，발명제비료미낭조독소 MC-LR 적면역층석쾌속검측잡，해잡검측양본중적미낭조독소 MC-LR 함량역치위2 ng·mL－1。
A colloidal gold immunoassay methodology was developed using the conjugate of the lab-made monoclonal antibody of diarrhetic shellfish poison,microcystin-LR (MC-LR ), and KLH as the labeled substrate. The conjugate was produced following the immune competition law.Colloidal gold with various particle sizes were prepared.The particle size of 30 nm was selected for labeling the antibody.The rapid detection was carried out on an immunochromatographic strip made of a nitrocellulose membrane coated with the conjugate as the inspection band and the goat anti-mouse IgG as the quality control band. Without a need for additional equipment,this newly developed test strip could give a rapid,visual result in 3-5 minutes with a sensitivity and a low threshold MC-LR content of 2 ng·mL-1 .