CAJ | 학술논문

为了研究硝酸盐胁迫对玉米幼苗生长相关酶活性的影响，在前人研究工作的基础上以郑58、郑单958和昌7-2为研究材料，采用溶液培养方法，在不同浓度硝酸盐胁迫下，对3个品种的幼苗进行硝酸还原酶、谷氨酰胺合成酶、超氧化物歧化酶活性的测定。揭示了玉米幼苗在适宜、高、低浓度硝酸盐供应条件下3种酶活性的变化。结果显示：硝酸盐浓度2．00 mmol ／L 时，根系和叶片的超氧化物歧化酶活性较低，说明该浓度范围可以作为玉米幼苗适宜培养浓度。低（0．02，0．20 mmol ／L）、高（10．00，20．00 mmol ／L）浓度硝酸盐条件下，SOD 活性明显上升，杂交系郑单958体内 SOD 活性明显高于亲本郑58和昌7-2品种（P ＜0．05），说明郑单958品种在胁迫条件下有较高的自我保护作用。低硝酸盐浓度（0．02，0．20 mmol ／L）时，随着硝酸盐供应浓度的增加硝酸还原酶和谷氨酰胺合成酶活性增强；高硝酸盐浓度（10．00，20．00 mmol ／L）时，硝酸还原酶和谷氨酰胺合成酶活性下降。叶片 NR 活性下降程度小于根系，说明硝酸盐供应变化对根系影响较大。父本昌7-2对硝酸盐的还原作用明显弱于母本郑58和子代郑单958，尤其在胁迫条件下存在显著差异（P 值均＜0．05）。谷氨酰胺合成酶的变化趋势与硝酸还原酶活力基本相似。
위료연구초산염협박대옥미유묘생장상관매활성적영향，재전인연구공작적기출상이정58、정단958화창7-2위연구재료，채용용액배양방법，재불동농도초산염협박하，대3개품충적유묘진행초산환원매、곡안선알합성매、초양화물기화매활성적측정。게시료옥미유묘재괄의、고、저농도초산염공응조건하3충매활성적변화。결과현시：초산염농도2．00 mmol ／L 시，근계화협편적초양화물기화매활성교저，설명해농도범위가이작위옥미유묘괄의배양농도。저（0．02，0．20 mmol ／L）、고（10．00，20．00 mmol ／L）농도초산염조건하，SOD 활성명현상승，잡교계정단958체내 SOD 활성명현고우친본정58화창7-2품충（P ＜0．05），설명정단958품충재협박조건하유교고적자아보호작용。저초산염농도（0．02，0．20 mmol ／L）시，수착초산염공응농도적증가초산환원매화곡안선알합성매활성증강；고초산염농도（10．00，20．00 mmol ／L）시，초산환원매화곡안선알합성매활성하강。협편 NR 활성하강정도소우근계，설명초산염공응변화대근계영향교대。부본창7-2대초산염적환원작용명현약우모본정58화자대정단958，우기재협박조건하존재현저차이（P 치균＜0．05）。곡안선알합성매적변화추세여초산환원매활력기본상사。
In order to understand the effect of nitrate stress on nirtogen assimilation related enzyme activity of maize,on the basis of previous research,Zheng 58,Zhengdan 958 and Chang 7-2 as study materials,under the ni-trate stress of different concentrations,nitrate reductase,glutamine synthetase,measuring superoxides dismutase ac-tivity were analyzed using solution culture method.It was revealed that changes of three enzymes activity of maize seedlings at suitable,high,low concentrations of nitrate supply conditions.The results showed that SOD activity of roots and leaves were low when nitrate concentration was 2.00 mmol /L,indicating that the concentration range was suitable for maize seedlings.Low (0.02,0.20 mmol /L),high (1 0.00,20.00 mmol /L)at a concentration of nitrate conditions,SOD activity was significantly increased in vivo hybrids Zhengdan 958,which was significantly higher than SOD activity of the parental species Zheng 58 and Chang 7-2 (P <0.05).It showed that Zhengdan 958 varie-ties had a higher self-protection role under stress conditions.With the increasing concentration of nitrate supply NR and GS activity were increased at low concentration of nitric acid (0.02,0.20 mmol /L),At high nitrate concentra-tions (1 0.00,20.00 mmol /L),NR and GS activity were decreased.NR activity of leaves decreased less than the root,indicating greater nitrate supply changed in the root.The reduction of nitrate of paternal Chang 7-2 was signifi-cantly weaker than the female offspring Zheng 58 and Zhengdan 958 and in particular it was significant differences (P <0.05)under stress conditions.GS trends and NR enzyme activity were similar.