CAJ | 학술논문

目的：研制一种在离子交换色谱法蛋白质纯化中可对浓度进行监测、并可控制换样臂自动换样的实用装置，供研究室蛋白质纯化。方法：利用新型的紫外发光二极管为装置提供所需的280 nm光源，高灵敏度的S1336（滨松）光电检测器实时监测石英流通池内流过的纯化后的蛋白质液吸光情况，实现对蛋白质含量浓度的监测、控制。原料液蛋白浓度过低需更换进样时，装置会发出人声警报，自动停止工作。下位单片机通过激光红外测距传感器监测蛋白收集杯的液面高度和当前加样臂所处位置，如单杯集满，则自动移往下一空杯。仪器的主控计算机是三星S5PV210嵌入式系统，操作系统为Wince6.0，控制软件在Keil4.0、Visual Studio2005环境下编写。结果：经研究室实际使用，该装置可进行双通道紫外吸收值实时监测、曲线显示，一次最多可完成735 ml蛋白液的自动收集，通常5 h内无需人工值守换样。结论：仪器结构简单，运行可靠，满足预期目标要求，使用维护方便，性价比高。
목적：연제일충재리자교환색보법단백질순화중가대농도진행감측、병가공제환양비자동환양적실용장치，공연구실단백질순화。방법：이용신형적자외발광이겁관위장치제공소수적280 nm광원，고령민도적S1336（빈송）광전검측기실시감측석영류통지내류과적순화후적단백질액흡광정황，실현대단백질함량농도적감측、공제。원료액단백농도과저수경환진양시，장치회발출인성경보，자동정지공작。하위단편궤통과격광홍외측거전감기감측단백수집배적액면고도화당전가양비소처위치，여단배집만，칙자동이왕하일공배。의기적주공계산궤시삼성S5PV210감입식계통，조작계통위Wince6.0，공제연건재Keil4.0、Visual Studio2005배경하편사。결과：경연구실실제사용，해장치가진행쌍통도자외흡수치실시감측、곡선현시，일차최다가완성735 ml단백액적자동수집，통상5 h내무수인공치수환양。결론：의기결구간단，운행가고，만족예기목표요구，사용유호방편，성개비고。
To develop a low-cost, reliable, easy-to-maintain and practical instrument for protein purification. Some ultraviolet luminescent diode was used to provide 280 nm light source, and high-sensitivity S1336 photo-electric detector was employed for real-time monitoring of purified protein solution flowing through quartz cell to supervise the concentration of the protein. The instrument gave voice alarm and stopped working in case the protein concentration was less than the standard one. The lower SCM monitored the liquid level of the protein collecting cup and the position of loading arm through laser infrared distance sensor, so that a cup full of protein might be replaced by another empty cup. The instrument involved in Samsung S5PV210 embedded master computer, Wince6.0 operating system, Keil4.0 and VS2005. Trials proved that the instrument could perform real-time monitoring and curve display of dual-channel ultraviolet absorption, and could realize auto collection of 735 ml protein solution and up to 5-hour standby. The instrument developed has simple structure, high reliability and easy maintenance, and meets the desired require-ments.