CAJ | 학술논문

目的：观察雷帕霉素与环孢素对人类外周血CD4＋CD2＋5调节性T细胞（ Treg）体外增殖的影响。方法利用免疫磁珠法提取健康成人外周血CD4＋CD2＋5 Treg，流式细胞仪检测CD4＋CD2＋5 Treg纯度；将羧基荧光素乙酰乙酸进行标记的CD4＋CD2＋5 Treg分为A、B、C组，分别加入100 nmol／L雷帕霉素、410 nmol／L环孢素及空白培养基；培养7 d后上流式细胞仪，计算原代细胞所占比例。结果 A组原代细胞所占比例为8．8％±3．2％，B组为48．9％±7．0％，C组为33．2％±4．3％；A、B组分别与C组比较，P均＜0．05。结论雷帕霉素可促进CD4＋CD2＋5 Treg的体外增殖，有利于移植后患者免疫耐受的形成；而环孢素则抑制CD4＋CD2＋5 Treg的体外增殖，不利于免疫耐受的形成。
목적：관찰뢰파매소여배포소대인류외주혈CD4＋CD2＋5조절성T세포（ Treg）체외증식적영향。방법이용면역자주법제취건강성인외주혈CD4＋CD2＋5 Treg，류식세포의검측CD4＋CD2＋5 Treg순도；장최기형광소을선을산진행표기적CD4＋CD2＋5 Treg분위A、B、C조，분별가입100 nmol／L뢰파매소、410 nmol／L배포소급공백배양기；배양7 d후상류식세포의，계산원대세포소점비례。결과 A조원대세포소점비례위8．8％±3．2％，B조위48．9％±7．0％，C조위33．2％±4．3％；A、B조분별여C조비교，P균＜0．05。결론뢰파매소가촉진CD4＋CD2＋5 Treg적체외증식，유리우이식후환자면역내수적형성；이배포소칙억제CD4＋CD2＋5 Treg적체외증식，불리우면역내수적형성。
Objective To observe the influence of rapamycin and ciclosporin on the proliferation of human periheral blood CD4+CD2+5 regulatory T cells.Methods The CD4+CD2+5 regulatory T cells ( Treg cells) from human peripheral blood were isolated by immunomagnetic bead separation, the purity of CD4+CD2+5 Treg was measured by flow cytometry.We divid-ed the CD4+CD2+5 Treg into the groups A, B and C, which were added with 100 nmol/L rapamycin, 410 nmol/L cyclospo-rine and the blank culture medium.After 7 days′culture, we calculated the percentage of primary Treg cells of different groups by flow cytometry.Results The percentage of primary Treg cells in the groups A, B and C were 8.8%±3.2%, 48.9%±7.0%and 33.2%±4.3%, respectively.Significant difference was found when we compared groups A and B with group C, respectively ( all P <0.05).Conclusion Rapamycin can significantly promote the proliferation of CD4+CD2+5 Treg in vitro, and it promotes the formation of immune tolerance in patients after transplantation;but cyclosporine inhibits the proliferation of CD4+CD2+5 Treg and hampers the progression.