CAJ | 학술논문

目的：研究生长分化因子5（growth and differentiation 5，GDF5）对大鼠骨髓间质干细胞（bone marrow mesenchymal stem cells，BMSCs）生长分化的影响。方法分离和培养大鼠 BMSCs，培养液添加 GDF5（添加浓度分别为0、10、100 ng／mL），用细胞计数、MTT 比色检测细胞增殖变化，用甲苯胺蓝、阿辛蓝染色和 RT-PCR 检测细胞蛋白多糖（PG）和Ⅱ型胶原合成。结果在低糖 DMEM 中，GDF5组细胞数量显著增多，可以促进 BMSCs 生长。在内含地塞米松、胰岛素等的 DMEM／F12成软骨诱导液中，阿辛蓝浓度 OD 值在 GDF5组均随时间增加而增高，Ⅱ型胶原合成增多可以诱导 BMSCs 向软骨细胞分化。结论 GDF5在低糖 DMEM 中可促进 BMSCs 的增殖；在成软骨诱导体系中，可使单层培养的 BMSCs 聚集诱导其向软骨细胞分化，并形成软骨小结。
목적：연구생장분화인자5（growth and differentiation 5，GDF5）대대서골수간질간세포（bone marrow mesenchymal stem cells，BMSCs）생장분화적영향。방법분리화배양대서 BMSCs，배양액첨가 GDF5（첨가농도분별위0、10、100 ng／mL），용세포계수、MTT 비색검측세포증식변화，용갑분알람、아신람염색화 RT-PCR 검측세포단백다당（PG）화Ⅱ형효원합성。결과재저당 DMEM 중，GDF5조세포수량현저증다，가이촉진 BMSCs 생장。재내함지새미송、이도소등적 DMEM／F12성연골유도액중，아신람농도 OD 치재 GDF5조균수시간증가이증고，Ⅱ형효원합성증다가이유도 BMSCs 향연골세포분화。결론 GDF5재저당 DMEM 중가촉진 BMSCs 적증식；재성연골유도체계중，가사단층배양적 BMSCs 취집유도기향연골세포분화，병형성연골소결。
Objective To explore the effect of GDF5 on the differentiation of bone marrow stromal cells (BMSCs)into chondrocytes.Methods The methods of harvest and culture of BMSCs from rats were used;GDF5 was added in culture fluid,cell counting and MTT were used to test the cell proliferation;Toludine blue,alcian blue staining and RT-PCR were used to examine the synthesis of proteoglycan and collagen.Results GDF5 was showed to be promotor for BMSCS growth in low sugar DMEM′s fluid,re-sulting in that the number of BMSCs increased,logarithmic growth was in advance,cell aggregated fol-lowed the confluence,the absorbed optical density on cell growth enhanced.In the DMEM/F12 cultured fluid to induce the chondrogensis contained Dexamethasone,Insuline and so on,the effect of GDF5 was showed to induce the differentiation of BMSCs into chondrocytes.Conclusion GDF5 could promote the proliferation of BMSCs in the low sugar DMEM's fluid,make the aggregation of BMSCs in the system in-duced to chondrogensis and induce the BMSCs differentiation into chondrocyte and form the cartilage nodule.