CAJ | 학술논문

抗病毒药物具有广泛的应用，对人类和动物的健康有着重要的作用。但抗病毒药物紫外吸收差异大、极性分布广，要实现该类药物的同时分析较为困难。本实验以Click TE?Cys为固定相，结合电雾式检测（ CAD），建立了多种抗病毒药物同时分离分析的方法。考察了不同的检测器、色谱模式、色谱柱和流动相对抗病毒药物峰形、分离选择性及检测信号响应的影响。在优化的条件下定量分析了5种抗病毒药物，结果证明该方法具有较好的日内重复性（ RSD≤3?06％）和日间重复性（ RSD≤5?38％）。同时，该方法还具有较宽的线性范围（0?07～2?28 mg／mL）和较高的灵敏度（ LOQ≤0?04 mg／mL），可用于相关药物的定量、定性分析。
항병독약물구유엄범적응용，대인류화동물적건강유착중요적작용。단항병독약물자외흡수차이대、겁성분포엄，요실현해류약물적동시분석교위곤난。본실험이Click TE?Cys위고정상，결합전무식검측（ CAD），건립료다충항병독약물동시분리분석적방법。고찰료불동적검측기、색보모식、색보주화류동상대항병독약물봉형、분리선택성급검측신호향응적영향。재우화적조건하정량분석료5충항병독약물，결과증명해방법구유교호적일내중복성（ RSD≤3?06％）화일간중복성（ RSD≤5?38％）。동시，해방법환구유교관적선성범위（0?07～2?28 mg／mL）화교고적령민도（ LOQ≤0?04 mg／mL），가용우상관약물적정량、정성분석。
Antiviral drugs are widely used for human and animals. However, the analysis of the mixture of antiviral drugs is a challenge for high performance liquid chromatography, since some of the antiviral drugs have weak UV absorbance and poor retention in reversed phase liq?uid chromatography. A method of hydrophilic interaction liquid chromatography?charged aerosol detection ( HILIC?CAD) was optimized for the qualitative and quantitative analysis of five antivi?ral drugs. In this study, Click TE?Cys was used as the stationary phase and CAD was used as the detector. Various chromatographic conditions including the kind of detector, chromato?graphic mode, column and mobile phase composition were investigated. Compared to UV?Vis, more antiviral drugs could be detected by CAD, since it is a universal detector. HILIC mode is an alternative to reversed phase liquid chromatography mode. HILIC provides higher sensitivity and unique selectivity to target compounds. After the optimized parameters were obtained, the developed method was used for the quantitative analysis of the five antiviral drugs. As a result, the current method has good repeatability, a wide linear range (0?07-2?28 mg/mL) and good sensitivity ( LOQ≤0?04 mg/mL) . The RSDs of intra?day and inter?day peak areas were less than 3?06% and 5?38% respectively. The above results demonstrated that the current method is sen?sitive, robust and effective for the separation and determination of these five antiviral drugs.