CAJ | 학술논문

糖类化合物因其极性强，在反相色谱模式下保留较弱，因此常用亲水作用色谱（ HILIC）对其进行分离分析。本文以9种糖类化合物的混合物为研究对象，系统评价了其在Click TE?Cys亲水色谱柱上的保留行为，分别考察了流动相中有机相比例和盐浓度对其保留行为的影响。实验证明：9种糖类化合物按极性由小到大的顺序依次从Click TE?Cys色谱柱上被洗脱下来。随着有机相比例的增加，糖类化合物的保留增强；随着盐浓度的增加，除唾液酸外的糖类化合物的保留增强。用顶替?吸附液相相互作用模型模拟了糖类化合物在HILIC上的保留行为，采用保留方程ln k＝a＋bln CB＋cCB 描述HILIC的保留规律，对HILIC的保留值进行多元线性回归。结果表明糖类化合物在Click TE?Cys色谱柱上的保留行为符合HILIC的保留规律。
당류화합물인기겁성강，재반상색보모식하보류교약，인차상용친수작용색보（ HILIC）대기진행분리분석。본문이9충당류화합물적혼합물위연구대상，계통평개료기재Click TE?Cys친수색보주상적보류행위，분별고찰료류동상중유궤상비례화염농도대기보류행위적영향。실험증명：9충당류화합물안겁성유소도대적순서의차종Click TE?Cys색보주상피세탈하래。수착유궤상비례적증가，당류화합물적보류증강；수착염농도적증가，제타액산외적당류화합물적보류증강。용정체?흡부액상상호작용모형모의료당류화합물재HILIC상적보류행위，채용보류방정ln k＝a＋bln CB＋cCB 묘술HILIC적보류규률，대HILIC적보류치진행다원선성회귀。결과표명당류화합물재Click TE?Cys색보주상적보류행위부합HILIC적보류규률。
Because of the strong polarity of carbohydrate compounds which have weak reten?tion in reverse?phase liquid chromatography ( RPLC) , hydrophilic interaction liquid chromatog?raphy ( HILIC) is suitable for the separation and analysis of the carbohydrate compounds. In this study, nine carbohydrate compounds were selected as the test probes to systematically evaluate the retention behavior of the carbohydrate compounds on a Click TE?Cys column with zwitterionic stationary phase. And the effects of the ratio of organic phase and salt concentra?tion in mobile phase on the retention behavior were investigated as well. The experiments proved that the nine carbohydrate compounds could be eluted in the order of their polarities from the Click TE?Cys column. With the increase of organic phase ratio, the retention of carbo?hydrate compounds was enhanced. As the increase of salt concentration, the retention of the carbohydrate compounds increased except sialic acid. Using displacement?adsorption liquid phase interaction model to simulate the retention behavior of the carbohydrate compounds under HILIC, the retention behavior of HILIC was described by the retention equation of ln k=a+bln CB+cCB, and the retention values of HILIC were analyzed by multiple linear regression. The results demonstrated that the retention behavior of carbohydrate compounds on the Click TE?Cys was in accordance with the retention regularity of HILIC.