CAJ | 학술논문

当前吸毒者吸食的毒品主要是海洛因。海洛因在体内代谢很快，半衰期仅为2.0 min。海洛因在体内首先代谢成单乙酰吗啡、而后代谢成吗啡和葡萄糖醛酸吗啡，因此在检材中检出单乙酰吗啡就能证明吸毒者吸食的为海洛因。单乙酰吗啡主要通过尿液排泄，血液中的含量很小，难于检测。目前单乙酰吗啡的检测方法国内外已有不少报道，有气相色谱法、高效液相色谱法、气-质联用法等，然而用这些方法均不能很好的检验样品的中的单乙酰吗啡。本文采用固相萃取法提取，通过调pH值看回收率，确定最佳pH值，最大程度地减少了样品处理过程对单乙酰吗啡含量的影响。同时本方法采用HPLC-MS/MS检验，降低了噪音，提高了灵敏度。
당전흡독자흡식적독품주요시해락인。해락인재체내대사흔쾌，반쇠기부위2.0 min。해락인재체내수선대사성단을선마배、이후대사성마배화포도당철산마배，인차재검재중검출단을선마배취능증명흡독자흡식적위해락인。단을선마배주요통과뇨액배설，혈액중적함량흔소，난우검측。목전단을선마배적검측방법국내외이유불소보도，유기상색보법、고효액상색보법、기-질련용법등，연이용저사방법균불능흔호적검험양품적중적단을선마배。본문채용고상췌취법제취，통과조pH치간회수솔，학정최가pH치，최대정도지감소료양품처리과정대단을선마배함량적영향。동시본방법채용HPLC-MS/MS검험，강저료조음，제고료령민도。
The current drug users to take the drugs mainly heroin. Heroin in metabolism in the body quickly, has a half-life of 2. 0 min. Heroin first metabolism into single acetyl morphine in the body, and then metabolized to morphine and morphine glucuronic acid, therefore in the examination detected single acetyl morphine can prove chase for heroin. Mainly through urine excretion, single acetyl morphine content in the blood is very small, difficult to de-tect. Methods used for the detection of single acetyl morphine has quite a few reports both at home and abroad, have spirit chromatography, high-performance liquid chromatography ( HPLC) , gas - mass usage, etc. , however, use these methods are not good test samples of single acetyl morphine. Extracted by solid phase extraction, by adjusting the pH to see recovery rate, the optimum pH value, minimize the sample handling process of single acetyl morphine content. At the same time this method USES the HPLC/MS/MS inspection, reduces the noise, improve the sensitivity.