CAJ | 학술논문

利用－NCO和－OH的加成反应，通过简单的两步反应将木糖醇和麦芽糖醇成功地键合于硅胶表面，制备了两种新型糖醇类亲水作用色谱固定相。流动相中乙腈含量对保留的影响曲线表明，这两种糖醇固定相具有典型的亲水作用色谱固定相性质，对极性和亲水性化合物有很强的保留作用。利用这两种固定相成功分离了水溶性维生素、水杨酸及其类似物、碱基及其相应的核苷和淫羊藿苷类似物等模型混合物，同时糖醇固定相展现了新颖的选择性，特别是相对于线形的木糖醇键合固定相，非线形的麦芽糖醇键合固定相表现出了对糖基的独特保留能力。此外，缓冲盐的pH和浓度对保留的影响表明静电作用在这两种糖醇固定相的保留机理中也发挥着一定的作用。本文所发展的糖醇类固定相具有良好的分离性能，有望在亲水作用色谱分离领域发挥潜在的应用价值。
이용－NCO화－OH적가성반응，통과간단적량보반응장목당순화맥아당순성공지건합우규효표면，제비료량충신형당순류친수작용색보고정상。류동상중을정함량대보류적영향곡선표명，저량충당순고정상구유전형적친수작용색보고정상성질，대겁성화친수성화합물유흔강적보류작용。이용저량충고정상성공분리료수용성유생소、수양산급기유사물、감기급기상응적핵감화음양곽감유사물등모형혼합물，동시당순고정상전현료신영적선택성，특별시상대우선형적목당순건합고정상，비선형적맥아당순건합고정상표현출료대당기적독특보류능력。차외，완충염적pH화농도대보류적영향표명정전작용재저량충당순고정상적보류궤리중야발휘착일정적작용。본문소발전적당순류고정상구유량호적분리성능，유망재친수작용색보분리영역발휘잠재적응용개치。
New types of stationary phases for hydrophilic interaction liquid chromatography ( HILIC ) with unique selectivity are very important for the separation of various polar and hydrophilic analytes. Two novel HILIC stationary phases based on sugar alcohol modified silica were synthesized by a simple two?step reaction in which xylitol and maltitol were bonded onto the surface of silica particles via the addition reaction between -NCO and -OH. The effect of acetonitrile content on the retention indicated that the two stationary phases were of typical HILIC character and exhibited strong retention for polar and hydrophilic analytes. They succee?ded in the separation of a wide range of polar and hydrophilic analytes including water soluble vitamins, salicylic acid and its analogues, nucleic acid bases and nucleosides, and icariin and its analogues with unique selectivity. Especially, the maltitol stationary phase showed unique selectivity on glycosyl group, compared to xylitol stationary phase. Furthermore, the effects of buffer pH as well as salt concentration on the retention indicated that electrostatic interaction played an important role in the separation mechanism of the two stationary phases. For sure, the efficient stationary phases are of great potential applications in HILIC.