CAJ | 학술논문

采用斑茅纤维为原料，用2％NaOH 和1％H2 O2进行预处理，探讨纤维素酶液态发酵法糖化工艺的优化，分析糖化材料、时间、pH、纤维用量、温度和转速等单因素对纤维素产糖的影响。结果表明：糖化材料用2％NaOH 和1％H2 O2处理，反应时间在28～30 h，pH 在4．5～5．5，纤维用量在4～6 g，温度在50～55℃，转速约为180 r·min－1时斑茅纤维水解的效果最好。通过正交试验设计确定最佳糖化条件：糖化温度52℃、时间30 h、纤维用量5 g、pH 6．0，糖化率达到45．21％。
채용반모섬유위원료，용2％NaOH 화1％H2 O2진행예처리，탐토섬유소매액태발효법당화공예적우화，분석당화재료、시간、pH、섬유용량、온도화전속등단인소대섬유소산당적영향。결과표명：당화재료용2％NaOH 화1％H2 O2처리，반응시간재28～30 h，pH 재4．5～5．5，섬유용량재4～6 g，온도재50～55℃，전속약위180 r·min－1시반모섬유수해적효과최호。통과정교시험설계학정최가당화조건：당화온도52℃、시간30 h、섬유용량5 g、pH 6．0，당화솔체도45．21％。
Liquid digestion of Erianthus arundinaceum fibers in the solution containing 2%NaOH and 1%H2 O2 to achieve the desired cellulase saccharification was studied.Effect of the substrates,digestion time,pH,fiber content,temperature and agitation speed on the process were analyzed.The conditions studied for the fiber hydrolysis included the applications of 2% NaOH and 1%H2 O2 ,28 to 30 h of reaction time,pH4.5 to 5.5,4 to 6 gof fiber,temperatures between 50℃ and 55℃,and agitation speeds of approximately 180 r · min-1 .The orthogonal experiment indicated the optimal conditions to reach a saccharification rate of 45.21% were 30 h,pH 6.0,5 g fiber and 52℃.