CAJ | 학술논문

目的：研究右美托咪定与维生素C单独或联合使用对小鼠小肠缺血再灌注损伤（ I／R）的保护作用及可能机制。方法雄性小鼠50只，随机分为5组（ n＝10）：假手术组（ sham组，暴露肠系膜上动脉，不结扎），肠缺血再灌注组（I／R组），右美托咪定组（DEX组，提前30 min注射DEX），维生素C组（VC组，提前10 min注射VC），右美托咪定联合维生素C组（ DEX＋VC组，提前30 min注射DEX，提前10 min注射VC）。缺血20 min再灌注24 h后取小肠组织，苏木精－伊红染色观察小肠黏膜的损伤情况，免疫组化法检测小肠黏膜中白细胞介素－6（IL－6）、肿瘤坏死因子α（TNF－α）和caspase－3的表达变化并做chiu′s评分。结果 DEX、VC、DEX＋VC组小肠黏膜的chiu′s评分和IL－6、TNF－α、caspase－3的表达较I／R组降低（P＜0．05），而较sham组升高（P＜0．05）；DEX组和VC组差异无统计学意义（ P＞0．05），DEX ＋VC组小肠黏膜的chiu′s评分和IL－6、TNF－α、caspase－3的表达较DEX、VC组降低（P＜0．05）。结论右美托咪定、维生素C对小鼠小肠缺血再灌注损伤有保护作用，两者联用有协同作用，且保护作用与IL－6，TNF－α和caspase－3有关。
목적：연구우미탁미정여유생소C단독혹연합사용대소서소장결혈재관주손상（ I／R）적보호작용급가능궤제。방법웅성소서50지，수궤분위5조（ n＝10）：가수술조（ sham조，폭로장계막상동맥，불결찰），장결혈재관주조（I／R조），우미탁미정조（DEX조，제전30 min주사DEX），유생소C조（VC조，제전10 min주사VC），우미탁미정연합유생소C조（ DEX＋VC조，제전30 min주사DEX，제전10 min주사VC）。결혈20 min재관주24 h후취소장조직，소목정－이홍염색관찰소장점막적손상정황，면역조화법검측소장점막중백세포개소－6（IL－6）、종류배사인자α（TNF－α）화caspase－3적표체변화병주chiu′s평분。결과 DEX、VC、DEX＋VC조소장점막적chiu′s평분화IL－6、TNF－α、caspase－3적표체교I／R조강저（P＜0．05），이교sham조승고（P＜0．05）；DEX조화VC조차이무통계학의의（ P＞0．05），DEX ＋VC조소장점막적chiu′s평분화IL－6、TNF－α、caspase－3적표체교DEX、VC조강저（P＜0．05）。결론우미탁미정、유생소C대소서소장결혈재관주손상유보호작용，량자련용유협동작용，차보호작용여IL－6，TNF－α화caspase－3유관。
Objective To investigate the protective effects of dexmedetomidine ( DEX) , vitamin C ( VC) or both a-gainst intestinal ischemia/reperfusion in mice and possible mechanism .Methods A total of 50 mice were randomly di-vided into five groups (n=10) according to their treatments:a sham group (in which the superior mesenteric artery was exposed without ligation ), an intestinal ischemia/reperfusion ( I/R) group ( which underwent ischemia for 20 min fol-lowed by reperfusion for 24 h), a dexmedetomidine (DEX) group (which received DEX 30 min before intestinal I/R, followed by ischemia for 20 min and consequently reperfusion for 24 h) , a vitamin C ( VC) group ( which received vita-min C 10 min before intestinal I/R, followed by ischemia for 20 min and consequently reperfusion for 24 h) , and a com-bined treatment ( DEX+VC) group ( which were injected with DEX and VC 30 min and 10 min before intestinal I/R re-spectively, followed by ischemia for 20 min and consequently reperfusion for 24 h).The pathological changes of the small intestinal mucosa were observed by HE staining .The amounts of IL-6, TNF-αand caspase-3 were detected by im-munohistochemistry and analyzed by Chiu′s scoring assessment .Results Groups DEX, VC, and DEX+VC produced the scores of Chiu′s ratings and the amounts of IL -6, TNF-αand caspase-3 which were lower than Group I/R (P<0.05), but higher than Group sham (P<0.05).But no statistical difference was determined between Groups DEX and VC (P>0.05).Group DEX+VC produced reduced Chiu′s scores and the amounts of IL -6, TNF-αand caspase-3 when compared with Groups DEX and VC (P<0.05).Conclusion Dexmedetomidine and vitamin C attenuate the in-testinal ischemia/reperfusion injury in mice , which can result in synergism related with IL -6, TNF-αand caspase-3.