江苏科技信息
江囌科技信息
강소과기신식
Jiangsu Science & Technology Information
2015年
24期
33-35
,共3页
抗结核药%肝损伤%TNF-α%NF-κB
抗結覈藥%肝損傷%TNF-α%NF-κB
항결핵약%간손상%TNF-α%NF-κB
antituberculosis drugs%liver injury%TNF-α%NF-κB
为探讨阿魏酸钠（SF）对抗结核药物肝损伤的保护作用机制，文章将60只SD雄性大鼠随机分为6组，即对照组、模型组（异烟肼+利福平）、SF低、中、高剂量组（30g/kg，60g/kg，120g/kg）和硫普罗宁组（60 mg/kg），给药8周。分别测定大鼠血清谷丙转氨酶（ALT）、谷草转氨酶（AST）、白介素-1β（IL-1β）、IL-6和肿瘤坏死因子-α（TNF-α）水平。采用Western Blotting方法测定大鼠肝脏TNF-α和核转录因子-κB（NF-κB）蛋白表达水平。与模型组比较，SF中高剂量组均显著降低血清ALT，AST，IL-1β，IL-6和TNF-α水平，并降低肝脏TNF-α和NF-κB蛋白表达水平。结果证明，SF对抗结核药物肝损伤的保护作用机制可能与抑制NF-κB表达有关。
為探討阿魏痠鈉（SF）對抗結覈藥物肝損傷的保護作用機製，文章將60隻SD雄性大鼠隨機分為6組，即對照組、模型組（異煙肼+利福平）、SF低、中、高劑量組（30g/kg，60g/kg，120g/kg）和硫普囉寧組（60 mg/kg），給藥8週。分彆測定大鼠血清穀丙轉氨酶（ALT）、穀草轉氨酶（AST）、白介素-1β（IL-1β）、IL-6和腫瘤壞死因子-α（TNF-α）水平。採用Western Blotting方法測定大鼠肝髒TNF-α和覈轉錄因子-κB（NF-κB）蛋白錶達水平。與模型組比較，SF中高劑量組均顯著降低血清ALT，AST，IL-1β，IL-6和TNF-α水平，併降低肝髒TNF-α和NF-κB蛋白錶達水平。結果證明，SF對抗結覈藥物肝損傷的保護作用機製可能與抑製NF-κB錶達有關。
위탐토아위산납（SF）대항결핵약물간손상적보호작용궤제，문장장60지SD웅성대서수궤분위6조，즉대조조、모형조（이연정+리복평）、SF저、중、고제량조（30g/kg，60g/kg，120g/kg）화류보라저조（60 mg/kg），급약8주。분별측정대서혈청곡병전안매（ALT）、곡초전안매（AST）、백개소-1β（IL-1β）、IL-6화종류배사인자-α（TNF-α）수평。채용Western Blotting방법측정대서간장TNF-α화핵전록인자-κB（NF-κB）단백표체수평。여모형조비교，SF중고제량조균현저강저혈청ALT，AST，IL-1β，IL-6화TNF-α수평，병강저간장TNF-α화NF-κB단백표체수평。결과증명，SF대항결핵약물간손상적보호작용궤제가능여억제NF-κB표체유관。
In order to investigate the protective effects of sodium ferulate (SF) on the hepatic injury induced by antituberculosis drugs in rats,the paper divided randomly sixty male SD rats into 6 groups:normal group,model group (isoniazid and rifampin),SF (30g/kg,60g/kg,120g/kg)and tiopronin group (60mg/kg).One hour after the final treatment,the alanine aminotransferase (ALT),aspartate aminotransferase (AST),interleukin-1β (IL-1β),IL-6, tumor necrosis factor-α (TNF-α)levels in serum were measured.Expression of TNF-α and nuclear transcription factor-κB (NF-κB)protein levels were assayed by Western Blotting method.Compared to vehicle-model group,SF at medium and high doses significantly reduced serum ALT, AST,IL-1β,IL-6 and TNF-α levels,and down-regulated liver TNF-α and NF-κB protein levels.The results showed that the protective effects of SF on the hepatic injury induced by antituberculosis drugs in rats may be related to its inhibition of NF-κB.
