CAJ | 학술논문

目的：探讨具核梭杆菌（ Fn）感染与结直肠癌（ CRC）的相关性。方法对手术切除、经病理证实的101例CRC组织和配对正常组织进行实时荧光定量PCR（FQ－PCR）检测组织中Fn丰度。随机抽选其中22例CRC和配对正常组织进行荧光原位杂交，验证FQ－PCR的结果，分析Fn的富集度与临床病理指标的相关性。结果两检测方法均证实Fn在CRC组织中富集，CRC组织中Fn丰度显著高于配对正常组织者占87.1％（88／101），CRC组织Fn丰度归一化值为0.242±0.034，显著高于配对正常组织的0.050±0.017（P＝0.000）。 Fn的高丰度与患者的性别、年龄、肿瘤位置、分化程度、浸润程度、Dukes分期等临床病理指标均无显著相关性（ P＞0.05），而与淋巴转移密切相关（ P＜0.05）。结论 Fn在CRC组织中富集，并有可能促进淋巴结转移。
목적：탐토구핵사간균（ Fn）감염여결직장암（ CRC）적상관성。방법대수술절제、경병리증실적101례CRC조직화배대정상조직진행실시형광정량PCR（FQ－PCR）검측조직중Fn봉도。수궤추선기중22례CRC화배대정상조직진행형광원위잡교，험증FQ－PCR적결과，분석Fn적부집도여림상병리지표적상관성。결과량검측방법균증실Fn재CRC조직중부집，CRC조직중Fn봉도현저고우배대정상조직자점87.1％（88／101），CRC조직Fn봉도귀일화치위0.242±0.034，현저고우배대정상조직적0.050±0.017（P＝0.000）。 Fn적고봉도여환자적성별、년령、종류위치、분화정도、침윤정도、Dukes분기등림상병리지표균무현저상관성（ P＞0.05），이여림파전이밀절상관（ P＜0.05）。결론 Fn재CRC조직중부집，병유가능촉진림파결전이。
Objective To investigate whether Fusobacterium nucleatum ( Fn) infection is associated with colorec-tal cancer ( CRC) .Methods The resected CRC ( pathologically confirmed) mass and matched normal samples from 101 patients were collected.Quantitative fluorescent polymerase chain reaction ( FQ-PCR) was applied to detect Fn in CRC versus matched normal tissues.Fluorescence in situ hybridization ( FISH) analysis was performed on 22 randomly selected CRC and matched samples to confirm FQ-PCR results.Abundance of Fn was analyzed for association with clinicopatho-logical parameters.Results Both analyses demonstrated that Fn was significantly over-represented in CRC.The rate of Fn over-abundance in CRC tissues versus that in matched normal tissues was 87.1%(88/101).The abundance of Fn was significantly greater in tumor tissues ( 0.242 ±0.034 ) than in matched normal tissues ( 0.050 ±0.017 ) ( P =0.000) .No significant association of Fn load with patients′gender, age, cancer location, stages and infiltration ( P>0.05) but with lymph node metastases (P=0.000) was observed.Conclusion Fn is enriched in the CRC tissues, which may be associated with CRC development and promote lymph node metastasis.