徐州医学院学报
徐州醫學院學報
서주의학원학보
Acta Academiae Medicinae Xuzhou
2015年
7期
451-455
,共5页
唐惠黎%王航%王远征%张咏梅
唐惠黎%王航%王遠徵%張詠梅
당혜려%왕항%왕원정%장영매
小鼠%母婴分离%内脏痛觉高敏
小鼠%母嬰分離%內髒痛覺高敏
소서%모영분리%내장통각고민
mice%maternal separation%visceral hypersensitivity
目的：探讨建立小鼠新生期母婴分离致慢性内脏痛觉高敏模型的可能性及意义。方法将新生小鼠随机分成2组，母婴分离组出生后第2～15天每天与母鼠分离3 h，对照组出生后不予处理。8周龄时进行行为学测试，测定评估内脏痛觉敏感性及对焦虑、抑郁行为的影响。结果与对照组比较：母婴分离组小鼠腹壁撤退反射评分增高（P＜0．01）、痛阈降低（P＜0．01）、腹外斜肌放电波幅明显升高（P＜0．05），表现出内脏痛觉高敏；在糖水偏好实验中未表现出行为差异（P＞0．05）；在旷场实验中中心区停留时间缩短（P＜0．05），出现焦虑行为；在强迫游泳实验中表现为不动时间延长（P＜0．01），存在行为绝望。结论新生期母婴分离能够导致小鼠发生成年后慢性内脏痛觉高敏，同时伴随焦虑和抑郁样症状。
目的：探討建立小鼠新生期母嬰分離緻慢性內髒痛覺高敏模型的可能性及意義。方法將新生小鼠隨機分成2組，母嬰分離組齣生後第2～15天每天與母鼠分離3 h，對照組齣生後不予處理。8週齡時進行行為學測試，測定評估內髒痛覺敏感性及對焦慮、抑鬱行為的影響。結果與對照組比較：母嬰分離組小鼠腹壁撤退反射評分增高（P＜0．01）、痛閾降低（P＜0．01）、腹外斜肌放電波幅明顯升高（P＜0．05），錶現齣內髒痛覺高敏；在糖水偏好實驗中未錶現齣行為差異（P＞0．05）；在曠場實驗中中心區停留時間縮短（P＜0．05），齣現焦慮行為；在彊迫遊泳實驗中錶現為不動時間延長（P＜0．01），存在行為絕望。結論新生期母嬰分離能夠導緻小鼠髮生成年後慢性內髒痛覺高敏，同時伴隨焦慮和抑鬱樣癥狀。
목적：탐토건립소서신생기모영분리치만성내장통각고민모형적가능성급의의。방법장신생소서수궤분성2조，모영분리조출생후제2～15천매천여모서분리3 h，대조조출생후불여처리。8주령시진행행위학측시，측정평고내장통각민감성급대초필、억욱행위적영향。결과여대조조비교：모영분리조소서복벽철퇴반사평분증고（P＜0．01）、통역강저（P＜0．01）、복외사기방전파폭명현승고（P＜0．05），표현출내장통각고민；재당수편호실험중미표현출행위차이（P＞0．05）；재광장실험중중심구정류시간축단（P＜0．05），출현초필행위；재강박유영실험중표현위불동시간연장（P＜0．01），존재행위절망。결론신생기모영분리능구도치소서발생성년후만성내장통각고민，동시반수초필화억욱양증상。
Objective To explore the possibility of establishment of a chronic visceral hypersensitivity model of mice induced by neonatal maternal separation and its meaning .Methods Neonatal mice were randomly divided into two groups.An half number of mice were subjected to 3 h of neonatal maternal separation per day from Days 2 to 15, while another half number of mice were set as controls .All mice underwent behavior test at eight weeks old , in order to evalu-ate their visceral sensitivity as well as anxiety and depression -like behavior induced by neonatal maternal separation . Results Compared with the control , neonatal maternal separation caused visceral hypersensitivity , especially higher ab-dominal withdrawal reflex (AWR) scores (P<0.01), lowered threshold of pain (P<0.01), and an elevated discharge rate in the musculus obliquus externus abdominis ( P<0 .05 ) .No significant behavior changes were found in the sugar preference test (P>0.05).After neonatal maternal separation , the mice presented anxiety and depression -like behav-ior, according to a shorter period of time in the central zone during the open field test (P<0.05) and an extended period of motionless in the forced swimming test ( P<0.01) respectively.Conclusion Neonatal maternal separation can lead to chronic visceral hypersensitivity in adult mice , accompanied with anxiety and depression -like behavior at the same time.
